biostudies-literatureUnknown28(4)Bruni SMicroanalysis techniques based on resonance Raman and reflection visible spectroscopy have been applied to the characterization of pigments responsible for the blue or violet coloration in flowers; in particular of <i>Lobelia erinus</i>, <i>Campanula portenschlagiana</i>, <i>Cineraria</i>, <i>Viola tricolor</i>, <i>Anemone coronaria</i>, <i>Agapanthus, Platycodon, Salvia farinacea, Plumbago capensis, Ceratostigma plumbaginoides</i>, <i>Commelina communis</i> and <i>Salvia patens</i>. The spectroscopic methods were applied both in vivo on the flower petals and in vitro on extracts obtained through a procedure based on SPE (solid-phase extraction) optimized for minimal quantities of vegetable raw material. Different patterns obtained for the Raman spectra have been correlated, also on the baMolecules (Basel, Switzerland)1709https://www.ebi.ac.uk/biostudies/studies/S-EPMC9959989biostudies-literatureResonance Raman and Visible Micro-Spectroscopy for the In-Vivo and In-Vitro Characterization of Anthocyanin-Based Pigments in Blue and Violet Flowers: A Comparison with HPLC-ESI- MS Analysis of the Extracts.PMC9959989Sironi MPieraccini SLongoni MMinzoni CZocca IBruni SBasili MfalseResonance Raman and Visible Micro-Spectroscopy for the In-Vivo and In-Vitro Characterization of Anthocyanin-Based Pigments in Blue and Violet Flowers: A Comparison with HPLC-ESI- MS Analysis of the Extracts.Microanalysis techniques based on resonance Raman and reflection visible spectroscopy have been applied to the characterization of pigments responsible for the blue or violet coloration in flowers; in particular of <i>Lobelia erinus</i>, <i>Campanula portenschlagiana</i>, <i>Cineraria</i>, <i>Viola tricolor</i>, <i>Anemone coronaria</i>, <i>Agapanthus, Platycodon, Salvia farinacea, Plumbago capensis, Ceratostigma plumbaginoides</i>, <i>Commelina communis</i> and <i>Salvia patens</i>. The spectroscopic methods were applied both in vivo on the flower petals and in vitro on extracts obtained through a procedure based on SPE (solid-phase extraction) optimized for minimal quantities of vegetable raw material. Different patterns obtained for the Raman spectra have been correlated, also on the ba2023-01-01T00:00:00Z2023 Feb2025-05-29T22:23:42.264Z2024-11-09T13:16:02.245ZS-EPMC99599893683869710.3390/molecules28041709