{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ayeni EA"],"funding":["National Science and Technology Resource Sharing Service Platform project of the Ministry of Science and Technology of China","CAS-TWAS President fellowship","Sichuan Science and Technology Program"],"pagination":["882"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9960078"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(4)"],"pubmed_abstract":["<i>Nigella glandulifera</i> is a traditional medicinal plant used to treat seizures, insomnia, and mental disorders among the Tibetan and Xinjiang people of China. Recent pharmacological research indicates that the seeds of this plant have a neuroprotective effect; however, the chemical components responsible for this effect are unknown. Monoamine oxidase B (MAO-B) has been recognized as a target for developing anti-Parkinson's disease drugs. In this work, MAO-B functionalized magnetic nanoparticles were used to enrich the enzyme's ligands in extracts of <i>N. glandulifera</i> seeds for rapid screening of MAO-B inhibitors coupled with HPLC-MS. Tauroside E and thymoquinone were found to inhibit the enzyme with IC<sub>50</sub> values of 35.85 μM and 25.54 μM, respectively. Both compounds exh"],"journal":["Plants (Basel, Switzerland)"],"pubmed_title":["Screening of Monoamine Oxidase Inhibitors from Seeds of <i>Nigella glandulifera</i> Freyn et Sint. by Ligand Fishing and Their Neuroprotective Activity."],"pmcid":["PMC9960078"],"funding_grant_id":["2022ZYD0032","E0117G1001"],"pubmed_authors":["Ayeni EA","Ma C","Hu Y","Zhang Y","Liao X","Bai X"],"additional_accession":[]},"is_claimable":false,"name":"Screening of Monoamine Oxidase Inhibitors from Seeds of <i>Nigella glandulifera</i> Freyn et Sint. by Ligand Fishing and Their Neuroprotective Activity.","description":"<i>Nigella glandulifera</i> is a traditional medicinal plant used to treat seizures, insomnia, and mental disorders among the Tibetan and Xinjiang people of China. Recent pharmacological research indicates that the seeds of this plant have a neuroprotective effect; however, the chemical components responsible for this effect are unknown. Monoamine oxidase B (MAO-B) has been recognized as a target for developing anti-Parkinson's disease drugs. In this work, MAO-B functionalized magnetic nanoparticles were used to enrich the enzyme's ligands in extracts of <i>N. glandulifera</i> seeds for rapid screening of MAO-B inhibitors coupled with HPLC-MS. Tauroside E and thymoquinone were found to inhibit the enzyme with IC<sub>50</sub> values of 35.85 μM and 25.54 μM, respectively. Both compounds exh","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-05-14T03:16:10.756Z","creation":"2024-11-05T18:13:43.704Z"},"accession":"S-EPMC9960078","cross_references":{"pubmed":["36840231"],"doi":["10.3390/plants12040882"]}}