biostudies-literatureAyeni EANational Science and Technology Resource Sharing Service Platform project of the Ministry of Science and Technology of ChinaCAS-TWAS President fellowshipSichuan Science and Technology Program882https://www.ebi.ac.uk/biostudies/studies/S-EPMC9960078biostudies-literatureUnknown12(4)<i>Nigella glandulifera</i> is a traditional medicinal plant used to treat seizures, insomnia, and mental disorders among the Tibetan and Xinjiang people of China. Recent pharmacological research indicates that the seeds of this plant have a neuroprotective effect; however, the chemical components responsible for this effect are unknown. Monoamine oxidase B (MAO-B) has been recognized as a target for developing anti-Parkinson's disease drugs. In this work, MAO-B functionalized magnetic nanoparticles were used to enrich the enzyme's ligands in extracts of <i>N. glandulifera</i> seeds for rapid screening of MAO-B inhibitors coupled with HPLC-MS. Tauroside E and thymoquinone were found to inhibit the enzyme with IC₅₀ values of 35.85 μM and 25.54 μM, respectively. Both compounds exhPlants (Basel, Switzerland)Screening of Monoamine Oxidase Inhibitors from Seeds of <i>Nigella glandulifera</i> Freyn et Sint. by Ligand Fishing and Their Neuroprotective Activity.PMC99600782022ZYD0032E0117G1001Ayeni EAMa CHu YZhang YLiao XBai XfalseScreening of Monoamine Oxidase Inhibitors from Seeds of <i>Nigella glandulifera</i> Freyn et Sint. by Ligand Fishing and Their Neuroprotective Activity.<i>Nigella glandulifera</i> is a traditional medicinal plant used to treat seizures, insomnia, and mental disorders among the Tibetan and Xinjiang people of China. Recent pharmacological research indicates that the seeds of this plant have a neuroprotective effect; however, the chemical components responsible for this effect are unknown. Monoamine oxidase B (MAO-B) has been recognized as a target for developing anti-Parkinson's disease drugs. In this work, MAO-B functionalized magnetic nanoparticles were used to enrich the enzyme's ligands in extracts of <i>N. glandulifera</i> seeds for rapid screening of MAO-B inhibitors coupled with HPLC-MS. Tauroside E and thymoquinone were found to inhibit the enzyme with IC₅₀ values of 35.85 μM and 25.54 μM, respectively. Both compounds exh2023-01-01T00:00:00Z2023 Feb2026-05-14T03:16:10.756Z2024-11-05T18:13:43.704ZS-EPMC99600783684023110.3390/plants12040882