biostudies-literatureIrenge LMBelgian Cooperation Agency of the ARES (Académie de Recherche et d'Enseignement Supérieur)Department Management of Scientific & Technological Research of Defence (IRSD-RSTD; Royal High Institute for Defence, Belgium).European Union's Horizon 2020 research and innovation program525https://www.ebi.ac.uk/biostudies/studies/S-EPMC9960421biostudies-literatureUnknown11(2)Multidrug-resistant (MDR) and extended spectrum β-lactamase (ESBL)-producing extra-intestinal <i>K. pneumoniae</i> are associated with increased morbidity and mortality. This study aimed to characterize the resistance and virulence profiles of extra-intestinal MDR ESBL-producing <i>K. pneumoniae</i> associated with infections at a tertiary hospital in South-Kivu province, DRC. Whole-genome sequencing (WGS) was carried out on 37 <i>K. pneumoniae</i> isolates displaying MDR and ESBL-producing phenotype. The assembled genomes were analysed for phylogeny, virulence factors and antimicrobial resistance genes (ARG) determinants. These isolates were compared to sub-Saharan counterparts. <i>K. pneumoniae</i> isolates displayed a high genetic variability with up to 16 sequence types (ST). AMR was wMicroorganismsGenomic Characterization of Multidrug-Resistant Extended Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> from Clinical Samples of a Tertiary Hospital in South Kivu Province, Eastern Democratic Republic of Congo.PMC9960421883285COOP-CONV-20-022HFM/18-10- 20192022Durant JFBonjean MAmbroise JIrenge LMGala JLBearzatto BfalseGenomic Characterization of Multidrug-Resistant Extended Spectrum β-Lactamase-Producing <i>Klebsiella pneumoniae</i> from Clinical Samples of a Tertiary Hospital in South Kivu Province, Eastern Democratic Republic of Congo.Multidrug-resistant (MDR) and extended spectrum β-lactamase (ESBL)-producing extra-intestinal <i>K. pneumoniae</i> are associated with increased morbidity and mortality. This study aimed to characterize the resistance and virulence profiles of extra-intestinal MDR ESBL-producing <i>K. pneumoniae</i> associated with infections at a tertiary hospital in South-Kivu province, DRC. Whole-genome sequencing (WGS) was carried out on 37 <i>K. pneumoniae</i> isolates displaying MDR and ESBL-producing phenotype. The assembled genomes were analysed for phylogeny, virulence factors and antimicrobial resistance genes (ARG) determinants. These isolates were compared to sub-Saharan counterparts. <i>K. pneumoniae</i> isolates displayed a high genetic variability with up to 16 sequence types (ST). AMR was w2023-01-01T00:00:00Z2023 Feb2025-04-21T17:28:53.382Z2024-12-04T12:37:52.037ZS-EPMC99604213683849010.3390/microorganisms11020525