biostudies-literatureLiu Xthe Research Project of Jinan Microecological Biomedicine Shandong Laboratory“20 Articles of Higher Education” Funding Project Independent Training Innovation Team of JinanUniversity of Bergenthe Special Foundation for Taishan Scholarsthe Clinical Research Center of Shandong UniversityNational Natural Science Foundation of ChinaCentral Guide Local Science and Technology Development Special Funds Projectsthe Department of Science &amp; Technology of Shandong Province147https://www.ebi.ac.uk/biostudies/studies/S-EPMC9960449biostudies-literatureUnknown21(1)<h4>Background</h4>Valtrate, a natural compound isolated from the root of Valeriana, exhibits antitumor activity in many cancers through different mechanisms. However, its efficacy for the treatment of glioblastoma (GBM), a tumor type with a poor prognosis, has not yet been rigorously investigated.<h4>Methods</h4>GBM cell lines were treated with valtrate and CCK-8, colony formation and EdU assays, flow cytometry, and transwell, 3D tumor spheroid invasion and GBM-brain organoid co-culture invasion assays were performed to assess properties of proliferation, viability, apoptosis and invasion/migration. RNA sequencing analysis on valtrate-treated cells was performed to identify putative target genes underlying the antitumor activity of the drug in GBM cells. Western blot analysis, immunofluorJournal of translational medicineValtrate, an iridoid compound in Valeriana, elicits anti-glioblastoma activity through inhibition of the PDGFRA/MEK/ERK signaling pathway.PMC9960449819723512020CXGC010903tshw201502056JNL-2022042CZR2019ZD332020SDUCRCB002tsqn201909173YDZX20210102019GXRC006JNL-2022003ALiu XLi XChen AHu YWen MYang NZhang XXue ZHuang BWang JLiu GfalseValtrate, an iridoid compound in Valeriana, elicits anti-glioblastoma activity through inhibition of the PDGFRA/MEK/ERK signaling pathway.<h4>Background</h4>Valtrate, a natural compound isolated from the root of Valeriana, exhibits antitumor activity in many cancers through different mechanisms. However, its efficacy for the treatment of glioblastoma (GBM), a tumor type with a poor prognosis, has not yet been rigorously investigated.<h4>Methods</h4>GBM cell lines were treated with valtrate and CCK-8, colony formation and EdU assays, flow cytometry, and transwell, 3D tumor spheroid invasion and GBM-brain organoid co-culture invasion assays were performed to assess properties of proliferation, viability, apoptosis and invasion/migration. RNA sequencing analysis on valtrate-treated cells was performed to identify putative target genes underlying the antitumor activity of the drug in GBM cells. Western blot analysis, immunofluor2023-01-01T00:00:00Z2023 Feb2026-05-28T19:01:49.53Z2025-02-19T00:13:34.29ZS-EPMC99604493682923510.1186/s12967-023-03984-0