biostudies-literatureWang LZaikang TongZhejiang Science and Technology Major Program on Agricultural New Variety BreedingJunhong ZhangNational Natural Science Foundation of China3829https://www.ebi.ac.uk/biostudies/studies/S-EPMC9960835biostudies-literatureUnknown24(4)Anthocyanins produce different-colored pigments in plant organs, which provide ornamental value. Thus, this study was conducted to understand the mechanism of anthocyanin synthesis in ornamental plants. <i>Phoebe bournei</i>, a Chinese specialty tree, has high ornamental and economic value due to its rich leaf color and diverse metabolic products. Here, the metabolic data and gene expression of red <i>P. bournei</i> leaves at the three developmental stages were evaluated to elucidate the color-production mechanism in the red-leaved <i>P. bournei</i> species. First, metabolomic analysis identified 34 anthocyanin metabolites showing high levels of cyanidin-3-O-glucoside (cya-3-O-glu) in the S1 stage, which may suggest that it is a characteristic metabolite associated with the red coloration International journal of molecular sciencesCyanidin-3-O-glucoside Contributes to Leaf Color Change by Regulating Two <i>bHLH</i> Transcription Factors in <i>Phoebe bournei</i>.PMC9960835Grant No. 2021C02070-102021C02070-10Grant No. 3217182832171828Song MWang QZhang JZhang YTong ZHan XYang QFu NWang LfalseCyanidin-3-O-glucoside Contributes to Leaf Color Change by Regulating Two <i>bHLH</i> Transcription Factors in <i>Phoebe bournei</i>.Anthocyanins produce different-colored pigments in plant organs, which provide ornamental value. Thus, this study was conducted to understand the mechanism of anthocyanin synthesis in ornamental plants. <i>Phoebe bournei</i>, a Chinese specialty tree, has high ornamental and economic value due to its rich leaf color and diverse metabolic products. Here, the metabolic data and gene expression of red <i>P. bournei</i> leaves at the three developmental stages were evaluated to elucidate the color-production mechanism in the red-leaved <i>P. bournei</i> species. First, metabolomic analysis identified 34 anthocyanin metabolites showing high levels of cyanidin-3-O-glucoside (cya-3-O-glu) in the S1 stage, which may suggest that it is a characteristic metabolite associated with the red coloration 2023-01-01T00:00:00Z2023 Feb2026-04-08T12:53:28.393Z2024-11-20T00:47:43.18ZS-EPMC99608353683524010.3390/ijms24043829