biostudies-literatureHolota MMINECOJunta de Comunidades de Castilla-la ManchaPolish National Agency for Academic ExchangeComunidad de Madrid and University of AlcaláNational Science Centre, PolandMinistry of Economy, Industry and CompetitivenessMinistry of UniversitiesNational Science CenterJunta de Comunidades de Castilla–la ManchaEuropean Regional Development Fund4076https://www.ebi.ac.uk/biostudies/studies/S-EPMC9960994biostudies-literatureUnknown24(4)Copper carbosilane metallodendrimers containing chloride ligands and nitrate ligands were mixed with commercially available conventional anticancer drugs, doxorubicin, methotrexate and 5-fluorouracil, for a possible therapeutic system. To verify the hypothesis that copper metallodendrimers can form conjugates with anticancer drugs, their complexes were biophysically characterized using zeta potential and zeta size methods. Next, to confirm the existence of a synergetic effect of dendrimers and drugs, in vitro studies were performed. The combination therapy has been applied in two cancer cell lines: MCF-7 (human breast cancer cell line) and HepG2 (human liver carcinoma cell line). The doxorubicin (DOX), methotrexate (MTX) and 5-fluorouracil (5-FU) were more effective against cancer cells whInternational journal of molecular sciencesCombination of Copper Metallodendrimers with Conventional Antitumor Drugs to Combat Cancer in In Vitro Models.PMC99609942017-T2/IND-5243CM/BG/2021-012018/31/F/NZ5/03454PPN/BIL/2018/1/00150NanoTENDOB2017/BMD-3733EUROPARTNERCTQ2017-86224-P685451SBPLY/17/180501/000358Bryszewska MGarcia-Gallego SHolota MMichlewska Sde la Mata FJDel Olmo NSOrtega PIonov MfalseCombination of Copper Metallodendrimers with Conventional Antitumor Drugs to Combat Cancer in In Vitro Models.Copper carbosilane metallodendrimers containing chloride ligands and nitrate ligands were mixed with commercially available conventional anticancer drugs, doxorubicin, methotrexate and 5-fluorouracil, for a possible therapeutic system. To verify the hypothesis that copper metallodendrimers can form conjugates with anticancer drugs, their complexes were biophysically characterized using zeta potential and zeta size methods. Next, to confirm the existence of a synergetic effect of dendrimers and drugs, in vitro studies were performed. The combination therapy has been applied in two cancer cell lines: MCF-7 (human breast cancer cell line) and HepG2 (human liver carcinoma cell line). The doxorubicin (DOX), methotrexate (MTX) and 5-fluorouracil (5-FU) were more effective against cancer cells wh2023-01-01T00:00:00Z2023 Feb2025-08-17T03:07:45.487Z2024-11-09T23:53:07.581ZS-EPMC99609943683548910.3390/ijms24044076