biostudies-literatureChristensen SSwedish Research Council for Environment Agricultural Sciences and Spatial PlanningMats Paulsson FoundationSwedish Research Council3973https://www.ebi.ac.uk/biostudies/studies/S-EPMC9961634biostudies-literatureUnknown24(4)Plant hemoglobins, often referred to as phytoglobins, play important roles in abiotic stress tolerance. Several essential small physiological metabolites can be bound to these heme proteins. In addition, phytoglobins can catalyze a range of different oxidative reactions in vivo. These proteins are often oligomeric, but the degree and relevance of subunit interactions are largely unknown. In this study, we delineate which residues are involved in dimer formation of a sugar beet phytoglobin type 1.2 (BvPgb1.2) using NMR relaxation experiments. <i>E. coli</i> cells harboring a phytoglobin expression vector were cultivated in isotope-labeled (²H, ¹³C and ¹⁵N) M9 medium. The triple-labeled protein was purified to homogeneity using two chromatographic steps. Two International journal of molecular sciencesConformational Dynamics of Phytoglobin BvPgb1.2 from <i>Beta vulgaris</i> ssp. <i>vulgaris</i>.PMC996163420192020-01852019-039962020-01855Akke MStenstrom OBulow LChristensen SfalseConformational Dynamics of Phytoglobin BvPgb1.2 from <i>Beta vulgaris</i> ssp. <i>vulgaris</i>.Plant hemoglobins, often referred to as phytoglobins, play important roles in abiotic stress tolerance. Several essential small physiological metabolites can be bound to these heme proteins. In addition, phytoglobins can catalyze a range of different oxidative reactions in vivo. These proteins are often oligomeric, but the degree and relevance of subunit interactions are largely unknown. In this study, we delineate which residues are involved in dimer formation of a sugar beet phytoglobin type 1.2 (BvPgb1.2) using NMR relaxation experiments. <i>E. coli</i> cells harboring a phytoglobin expression vector were cultivated in isotope-labeled (²H, ¹³C and ¹⁵N) M9 medium. The triple-labeled protein was purified to homogeneity using two chromatographic steps. Two 2023-01-01T00:00:00Z2023 Feb2025-04-05T07:24:31.748Z2025-04-05T07:24:31.748ZS-EPMC99616343683538110.3390/ijms24043973