{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Loh PS"],"funding":["Ministry of Education","National University of Singapore","Agency for Science, Technology and Research"],"pagination":["370"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9962178"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(2)"],"pubmed_abstract":["Covalently closed dumbbell-shaped DNA delivery vectors comprising the double-stranded gene(s) of interest and single-stranded hairpin loops on both ends represent a safe, stable and efficacious alternative to viral and other non-viral DNA-based vector systems. As opposed to plasmids and DNA minicircles, dumbbells can be conjugated via the loops with helper functions for targeted delivery or imaging. Here, we investigated the non-covalent linkage of tri-antennary N-acetylgalactosamine (GalNAc3) or a homodimer of a CD137/4-1BB-binding aptamer (aptCD137-2) to extended dumbbell vector loops via complementary oligonucleotides for targeted delivery into hepatocytes or nasopharyngeal cancer cells. Enlarging the dumbbell loop size from 4 to 71 nucleotides for conjugation did not impair gene expres"],"journal":["Pharmaceutics"],"pubmed_title":["Non-Covalent Linkage of Helper Functions to Dumbbell-Shaped DNA Vectors for Targeted Delivery."],"pmcid":["PMC9962178"],"funding_grant_id":["NUHSRO/2017/068/Bridging/06","H19H0G1006","T1-BSRG 2015-05","T1-2014Apr-02"],"pubmed_authors":["Loh PS","Patzel V"],"additional_accession":[]},"is_claimable":false,"name":"Non-Covalent Linkage of Helper Functions to Dumbbell-Shaped DNA Vectors for Targeted Delivery.","description":"Covalently closed dumbbell-shaped DNA delivery vectors comprising the double-stranded gene(s) of interest and single-stranded hairpin loops on both ends represent a safe, stable and efficacious alternative to viral and other non-viral DNA-based vector systems. As opposed to plasmids and DNA minicircles, dumbbells can be conjugated via the loops with helper functions for targeted delivery or imaging. Here, we investigated the non-covalent linkage of tri-antennary N-acetylgalactosamine (GalNAc3) or a homodimer of a CD137/4-1BB-binding aptamer (aptCD137-2) to extended dumbbell vector loops via complementary oligonucleotides for targeted delivery into hepatocytes or nasopharyngeal cancer cells. Enlarging the dumbbell loop size from 4 to 71 nucleotides for conjugation did not impair gene expres","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-25T23:05:11.696Z","creation":"2025-04-06T09:17:20.945Z"},"accession":"S-EPMC9962178","cross_references":{"pubmed":["36839697"],"doi":["10.3390/pharmaceutics15020370"]}}