{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Karuppiah V"],"funding":["China Agriculture Research System of MOF and MAR, the National Key R&D Program of China","Shanghai Agriculture Applied Technology Development Program","Shanghai Science and Technology Innovation Action Program of the Shanghai Science and Technology Commission","National Natural Science Foundation of China"],"pagination":["215"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9963050"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(2)"],"pubmed_abstract":["<i>Trichoderma</i> spp. are biological control agents extensively used against various plant pathogens. However, the key genes shared for the growth, development and biological activity are unclear. In this study, we explored the genes responsible for the growth and development of <i>T. asperellum</i> GDFS 1009 under liquid-shaking culture compared to solid-surface culture. Transcriptome analysis revealed 2744 differentially expressed genes, and RT-qPCR validation showed that the high-affinity methionine permease <i>MUP1</i> was the key gene for growth under different media. Deletion of the <i>MUP1</i> inhibited the transport of amino acids, especially methionine, thereby inhibiting mycelial growth and sporulation, whereas inhibition could be mitigated by adding methionine metabolites such"],"journal":["Journal of fungi (Basel, Switzerland)"],"pubmed_title":["Transcriptome Analysis of <i>T. asperellum</i> GDFS 1009 Revealed the Role of <i>MUP1</i> Gene on the Methionine-Based Induction of Morphogenesis and Biological Control Activity."],"pmcid":["PMC9963050"],"funding_grant_id":["21N41900200","31872015","2022-02-08-00-12-F0-01143","2017YFD0200403"],"pubmed_authors":["Li Y","Chen J","Karuppiah V","Zhang C","Liu T"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptome Analysis of <i>T. asperellum</i> GDFS 1009 Revealed the Role of <i>MUP1</i> Gene on the Methionine-Based Induction of Morphogenesis and Biological Control Activity.","description":"<i>Trichoderma</i> spp. are biological control agents extensively used against various plant pathogens. However, the key genes shared for the growth, development and biological activity are unclear. In this study, we explored the genes responsible for the growth and development of <i>T. asperellum</i> GDFS 1009 under liquid-shaking culture compared to solid-surface culture. Transcriptome analysis revealed 2744 differentially expressed genes, and RT-qPCR validation showed that the high-affinity methionine permease <i>MUP1</i> was the key gene for growth under different media. Deletion of the <i>MUP1</i> inhibited the transport of amino acids, especially methionine, thereby inhibiting mycelial growth and sporulation, whereas inhibition could be mitigated by adding methionine metabolites such","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-04T08:53:16.86Z","creation":"2025-04-04T08:53:16.86Z"},"accession":"S-EPMC9963050","cross_references":{"pubmed":["36836329"],"doi":["10.3390/jof9020215"]}}