{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["24(4)"],"submitter":["Xu M"],"pubmed_abstract":["Therapeutic antibodies-F(ab')<sub>2</sub> obtained from hyperimmune equine plasma could treat emerging infectious diseases rapidly because of their high neutralization activity and high output. However, the small-sized F(ab')<sub>2</sub> is rapidly eliminated by blood circulation. This study explored PEGylation strategies to maximize the half-life of equine anti-SARS-CoV-2 specific F(ab')<sub>2</sub>. Equine anti-SARS-CoV-2 specific F(ab')<sub>2</sub> were combined with 10 KDa MAL-PEG-MAL in optimum conditions. Specifically, there were two strategies: Fab-PEG and Fab-PEG-Fab, F(ab')<sub>2</sub> bind to a PEG or two PEG, respectively. A single ion exchange chromatography step accomplished the purification of the products. Finally, the affinity and neutralizing activity was evaluated by ELIS"],"journal":["International journal of molecular sciences"],"pagination":["3387"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9963672"],"repository":["biostudies-literature"],"pubmed_title":["PEGylation Prolongs the Half-Life of Equine Anti-SARS-CoV-2 Specific F(ab')<sub>2</sub>."],"pmcid":["PMC9963672"],"pubmed_authors":["Mo R","Feng N","Xia X","Xu M","Wang T","Wang X","Yang S","Bi J","Zhao Y","Liang B","Yan F"],"additional_accession":[]},"is_claimable":false,"name":"PEGylation Prolongs the Half-Life of Equine Anti-SARS-CoV-2 Specific F(ab')<sub>2</sub>.","description":"Therapeutic antibodies-F(ab')<sub>2</sub> obtained from hyperimmune equine plasma could treat emerging infectious diseases rapidly because of their high neutralization activity and high output. However, the small-sized F(ab')<sub>2</sub> is rapidly eliminated by blood circulation. This study explored PEGylation strategies to maximize the half-life of equine anti-SARS-CoV-2 specific F(ab')<sub>2</sub>. Equine anti-SARS-CoV-2 specific F(ab')<sub>2</sub> were combined with 10 KDa MAL-PEG-MAL in optimum conditions. Specifically, there were two strategies: Fab-PEG and Fab-PEG-Fab, F(ab')<sub>2</sub> bind to a PEG or two PEG, respectively. A single ion exchange chromatography step accomplished the purification of the products. Finally, the affinity and neutralizing activity was evaluated by ELIS","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-21T14:37:00.016Z","creation":"2025-04-21T14:37:00.016Z"},"accession":"S-EPMC9963672","cross_references":{"pubmed":["36834803"],"doi":["10.3390/ijms24043387"]}}