biostudies-literatureSlighoua MPrincess Nourah bint Abdulrahman University260https://www.ebi.ac.uk/biostudies/studies/S-EPMC9963972biostudies-literatureUnknown13(2)Parsley (<i>Petroselinum sativum</i> Hoffm.) is renowned for its ethnomedicinal uses including managing pain, wound, and dermal diseases. We previously highlighted the estrogenic and anti-inflammatory properties of parsley and profiled the phytochemistry of its polyphenolic fraction using HPLC-DAD. To extend our investigation, we here characterized the phytochemical composition of the hydro-ethanolic extract using LC-MS/MS and GC-MS upon silylation, and evaluated the antioxidant, analgesic, antimicrobial, and wound healing activities of its hydro-ethanolic and polyphenolic fraction. The antioxidant property was assessed using FRAP, DPPH, and TAC assays. The antimicrobial activity was tested against four wound infectious microbes (<i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i> MetabolitesLC-MS/MS and GC/MS Profiling of <i>Petroselinum sativum</i> Hoffm. and Its Topical Application on Burn Wound Healing and Related Analgesic Potential in Rats.PMC9963972PNURSP2023R141Amrati FESlighoua MMoussaid FZDrioiche AConte RBari AMahdi IHousseini AIKamaly OASaleh ABousta DfalseLC-MS/MS and GC/MS Profiling of <i>Petroselinum sativum</i> Hoffm. and Its Topical Application on Burn Wound Healing and Related Analgesic Potential in Rats.Parsley (<i>Petroselinum sativum</i> Hoffm.) is renowned for its ethnomedicinal uses including managing pain, wound, and dermal diseases. We previously highlighted the estrogenic and anti-inflammatory properties of parsley and profiled the phytochemistry of its polyphenolic fraction using HPLC-DAD. To extend our investigation, we here characterized the phytochemical composition of the hydro-ethanolic extract using LC-MS/MS and GC-MS upon silylation, and evaluated the antioxidant, analgesic, antimicrobial, and wound healing activities of its hydro-ethanolic and polyphenolic fraction. The antioxidant property was assessed using FRAP, DPPH, and TAC assays. The antimicrobial activity was tested against four wound infectious microbes (<i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i> 2023-01-01T00:00:00Z2023 Feb2025-04-04T08:52:51.081Z2025-04-04T08:52:51.081ZS-EPMC99639723683787910.3390/metabo13020260