{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Guegan H"],"funding":["Centre Hospitalier Universitaire de Rennes","Institut de Parasitologie de l'Ouest"],"pagination":["134"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9965090"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(2)"],"pubmed_abstract":["<i>Scedosporium apiospermum</i> is a saprophytic filamentous fungus involved in human infections, of which the virulence factors that contribute to pathogenesis are still poorly characterized. In particular, little is known about the specific role of dihydroxynaphtalene (DHN)-melanin, located on the external layer of the conidia cell wall. We previously identified a transcription factor, PIG1, which may be involved in DHN-melanin biosynthesis. To elucidate the role of PIG1 and DHN-melanin in <i>S. apiospermum</i>, a CRISPR-Cas9-mediated <i>PIG1</i> deletion was carried out from two parental strains to evaluate its impact on melanin biosynthesis, conidia cell-wall assembly, and resistance to stress, including the ability to survive macrophage engulfment. Δ<i>PIG1</i> mutants did not produce"],"journal":["Journal of fungi (Basel, Switzerland)"],"pubmed_title":["Deciphering the Role of PIG1 and DHN-Melanin in <i>Scedosporium apiospermum</i> Conidia."],"pmcid":["PMC9965090"],"funding_grant_id":["ATCF 2018-2022","#IPO2019"],"pubmed_authors":["Sergent O","Ravenel K","Dion S","Desvillechabrol D","Giraud S","Poirier W","Gastebois A","Gangneux JP","Guegan H","Gallais I","Delabarre A","Pinson X"],"additional_accession":[]},"is_claimable":false,"name":"Deciphering the Role of PIG1 and DHN-Melanin in <i>Scedosporium apiospermum</i> Conidia.","description":"<i>Scedosporium apiospermum</i> is a saprophytic filamentous fungus involved in human infections, of which the virulence factors that contribute to pathogenesis are still poorly characterized. In particular, little is known about the specific role of dihydroxynaphtalene (DHN)-melanin, located on the external layer of the conidia cell wall. We previously identified a transcription factor, PIG1, which may be involved in DHN-melanin biosynthesis. To elucidate the role of PIG1 and DHN-melanin in <i>S. apiospermum</i>, a CRISPR-Cas9-mediated <i>PIG1</i> deletion was carried out from two parental strains to evaluate its impact on melanin biosynthesis, conidia cell-wall assembly, and resistance to stress, including the ability to survive macrophage engulfment. Δ<i>PIG1</i> mutants did not produce","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-19T07:34:11.978Z","creation":"2025-02-18T23:57:36.484Z"},"accession":"S-EPMC9965090","cross_references":{"pubmed":["36836250"],"doi":["10.3390/jof9020134"]}}