{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Somayajulu M"],"funding":["NEI NIH HHS","NIDDK NIH HHS","NIEHS NIH HHS"],"pagination":["3911"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9965133"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(4)"],"pubmed_abstract":["The purpose of this study is to test the effects of whole-body animal exposure to airborne particulate matter (PM) with an aerodynamic diameter of <10 μm (PM<sub>10</sub>) in the mouse cornea and in vitro. C57BL/6 mice were exposed to control or 500 µg/m<sup>3</sup> PM<sub>10</sub> for 2 weeks. In vivo, reduced glutathione (GSH) and malondialdehyde (MDA) were analyzed. RT-PCR and ELISA evaluated levels of nuclear factor erythroid 2-related factor 2 (Nrf2) signaling and inflammatory markers. SKQ1, a novel mitochondrial antioxidant, was applied topically and GSH, MDA and Nrf2 levels were tested. In vitro, cells were treated with PM<sub>10</sub> ± SKQ1 and cell viability, MDA, mitochondrial ROS, ATP and Nrf2 protein were tested. In vivo, PM<sub>10</sub> vs. control exposure significantly redu"],"journal":["International journal of molecular sciences"],"pubmed_title":["Airborne Exposure of the Cornea to PM<sub>10</sub> Induces Oxidative Stress and Disrupts Nrf2 Mediated Anti-Oxidant Defenses."],"pmcid":["PMC9965133"],"funding_grant_id":["R01 DK132065","R01 DK090313","P30EY04068","R01 EY016058","R01 DK126908","R21 ES017829"],"pubmed_authors":["Wright R","Croniger B","Zhang K","Pitchaikannu A","McClellan SA","Hazlett LD","Somayajulu M"],"additional_accession":[]},"is_claimable":false,"name":"Airborne Exposure of the Cornea to PM<sub>10</sub> Induces Oxidative Stress and Disrupts Nrf2 Mediated Anti-Oxidant Defenses.","description":"The purpose of this study is to test the effects of whole-body animal exposure to airborne particulate matter (PM) with an aerodynamic diameter of <10 μm (PM<sub>10</sub>) in the mouse cornea and in vitro. C57BL/6 mice were exposed to control or 500 µg/m<sup>3</sup> PM<sub>10</sub> for 2 weeks. In vivo, reduced glutathione (GSH) and malondialdehyde (MDA) were analyzed. RT-PCR and ELISA evaluated levels of nuclear factor erythroid 2-related factor 2 (Nrf2) signaling and inflammatory markers. SKQ1, a novel mitochondrial antioxidant, was applied topically and GSH, MDA and Nrf2 levels were tested. In vitro, cells were treated with PM<sub>10</sub> ± SKQ1 and cell viability, MDA, mitochondrial ROS, ATP and Nrf2 protein were tested. In vivo, PM<sub>10</sub> vs. control exposure significantly redu","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-25T17:09:57.666Z","creation":"2025-02-19T04:20:50.629Z"},"accession":"S-EPMC9965133","cross_references":{"pubmed":["36835320"],"doi":["10.3390/ijms24043911"]}}