biostudies-literatureSomayajulu MNEI NIH HHSNIDDK NIH HHSNIEHS NIH HHS3911https://www.ebi.ac.uk/biostudies/studies/S-EPMC9965133biostudies-literatureUnknown24(4)The purpose of this study is to test the effects of whole-body animal exposure to airborne particulate matter (PM) with an aerodynamic diameter of <10 μm (PM₁₀) in the mouse cornea and in vitro. C57BL/6 mice were exposed to control or 500 µg/m³ PM₁₀ for 2 weeks. In vivo, reduced glutathione (GSH) and malondialdehyde (MDA) were analyzed. RT-PCR and ELISA evaluated levels of nuclear factor erythroid 2-related factor 2 (Nrf2) signaling and inflammatory markers. SKQ1, a novel mitochondrial antioxidant, was applied topically and GSH, MDA and Nrf2 levels were tested. In vitro, cells were treated with PM₁₀ ± SKQ1 and cell viability, MDA, mitochondrial ROS, ATP and Nrf2 protein were tested. In vivo, PM₁₀ vs. control exposure significantly reduInternational journal of molecular sciencesAirborne Exposure of the Cornea to PM₁₀ Induces Oxidative Stress and Disrupts Nrf2 Mediated Anti-Oxidant Defenses.PMC9965133R01 DK132065R01 DK090313P30EY04068R01 EY016058R01 DK126908R21 ES017829Wright RCroniger BZhang KPitchaikannu AMcClellan SAHazlett LDSomayajulu MfalseAirborne Exposure of the Cornea to PM₁₀ Induces Oxidative Stress and Disrupts Nrf2 Mediated Anti-Oxidant Defenses.The purpose of this study is to test the effects of whole-body animal exposure to airborne particulate matter (PM) with an aerodynamic diameter of <10 μm (PM₁₀) in the mouse cornea and in vitro. C57BL/6 mice were exposed to control or 500 µg/m³ PM₁₀ for 2 weeks. In vivo, reduced glutathione (GSH) and malondialdehyde (MDA) were analyzed. RT-PCR and ELISA evaluated levels of nuclear factor erythroid 2-related factor 2 (Nrf2) signaling and inflammatory markers. SKQ1, a novel mitochondrial antioxidant, was applied topically and GSH, MDA and Nrf2 levels were tested. In vitro, cells were treated with PM₁₀ ± SKQ1 and cell viability, MDA, mitochondrial ROS, ATP and Nrf2 protein were tested. In vivo, PM₁₀ vs. control exposure significantly redu2023-01-01T00:00:00Z2023 Feb2025-04-25T17:09:57.666Z2025-02-19T04:20:50.629ZS-EPMC99651333683532010.3390/ijms24043911