biostudies-literatureAbdallah BMDeputyship for Research & Innovation, Ministry Of Education in Saudi Arabia1536https://www.ebi.ac.uk/biostudies/studies/S-EPMC9965404biostudies-literatureUnknown28(4)<i>Trichophyton rubrum</i> is the most common dermatophyte, and can cause cutaneous infections in humans and animals (dermatophytosis). In this study, we investigated the anti-dermatophytic potential of green synthesized silver nanoparticles using <i>Achillea santolina</i> extract (AS-AgNPs) in an in vitro and in vivo rat model of dermal <i>T. rubrum</i> dermatophytosis (TRD). The green synthesis of AS-AgNPs was performed using <i>A. santolina</i> extract and characterized by UV-VIS spectroscopy, zeta potential, imaging (transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Energy dispersive X-ray analysis (EDX). The antifungal activity of AS-AgNPs was determined by the broth microdilution method, conidial germination, and hyphaMolecules (Basel, Switzerland)Potential Treatment of Dermatophyte <i>Trichophyton rubrum</i> in Rat Model Using Topical Green Biosynthesized Silver Nanoparticles with <i>Achillea santolina</i> Extract.PMC9965404INST175Rajendran PAli EMAbdallah BMfalsePotential Treatment of Dermatophyte <i>Trichophyton rubrum</i> in Rat Model Using Topical Green Biosynthesized Silver Nanoparticles with <i>Achillea santolina</i> Extract.<i>Trichophyton rubrum</i> is the most common dermatophyte, and can cause cutaneous infections in humans and animals (dermatophytosis). In this study, we investigated the anti-dermatophytic potential of green synthesized silver nanoparticles using <i>Achillea santolina</i> extract (AS-AgNPs) in an in vitro and in vivo rat model of dermal <i>T. rubrum</i> dermatophytosis (TRD). The green synthesis of AS-AgNPs was performed using <i>A. santolina</i> extract and characterized by UV-VIS spectroscopy, zeta potential, imaging (transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Energy dispersive X-ray analysis (EDX). The antifungal activity of AS-AgNPs was determined by the broth microdilution method, conidial germination, and hypha2023-01-01T00:00:00Z2023 Feb2025-04-26T00:05:52.413Z2025-04-06T09:35:04.538ZS-EPMC99654043683853110.3390/molecules28041536