biostudies-literatureSanchez-Lozano IConsejo Nacional de Ciencia y TecnologíaCONACYTInstituto Politécnico Nacional4249https://www.ebi.ac.uk/biostudies/studies/S-EPMC9965525biostudies-literatureUnknown24(4)In marine environments, biofilm can cause negative impacts, including the biofouling process. In the search for new non-toxic formulations that inhibit biofilm, biosurfactants (BS) produced by the genus <i>Bacillus</i> have demonstrated considerable potential. To elucidate the changes that BS from <i>B. niabensis</i> promote in growth inhibition and biofilm formation, this research performed a nuclear magnetic resonance (NMR) metabolomic profile analysis to compare the metabolic differences between planktonic cells and biofilms of <i>Pseudomonas stutzeri</i>, a pioneer fouling bacteria. The multivariate analysis showed a clear separation between groups with a higher concentration of metabolites in the biofilm than in planktonic cells of <i>P. stutzeri</i>. When planktonic and biofilm stageInternational journal of molecular sciencesMetabolomic Insights of Biosurfactant Activity from <i>Bacillus niabensis</i> against Planktonic Cells and Biofilm of <i>Pseudomonas stutzeri</i> Involved in Marine Biofouling.PMC9965525SIP-20221626SIP-20221829SIP-20221829, SIP-20221626, SIP-20221652SIP-20221652A1-S-14968Cruz-Narvaez YBand-Schmidt CJBecerra-Martinez EHernandez-Guerrero CJSanchez-Lozano IMunoz-Cruz LCHellio CfalseMetabolomic Insights of Biosurfactant Activity from <i>Bacillus niabensis</i> against Planktonic Cells and Biofilm of <i>Pseudomonas stutzeri</i> Involved in Marine Biofouling.In marine environments, biofilm can cause negative impacts, including the biofouling process. In the search for new non-toxic formulations that inhibit biofilm, biosurfactants (BS) produced by the genus <i>Bacillus</i> have demonstrated considerable potential. To elucidate the changes that BS from <i>B. niabensis</i> promote in growth inhibition and biofilm formation, this research performed a nuclear magnetic resonance (NMR) metabolomic profile analysis to compare the metabolic differences between planktonic cells and biofilms of <i>Pseudomonas stutzeri</i>, a pioneer fouling bacteria. The multivariate analysis showed a clear separation between groups with a higher concentration of metabolites in the biofilm than in planktonic cells of <i>P. stutzeri</i>. When planktonic and biofilm stage2023-01-01T00:00:00Z2023 Feb2026-04-08T12:51:50.423Z2024-11-20T00:47:30.147ZS-EPMC99655253683566210.3390/ijms24044249