{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bartosh AV"],"funding":["Ministry of Science and Higher Education of the Russian Federation","Russian Science Foundation"],"pagination":["333"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9965744"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(2)"],"pubmed_abstract":["Changes in the limits of detection (LODs) for a multiplex lateral flow immunoassay (LFIA) caused by different locations of the binding zone on the test strips were studied. Due to the non-equilibrium conditions of the immune reactions in LFIAs, their analytical parameters are susceptible to the binding constants of antigen-antibody reactions and assay duration. Consequently, the integration of several tests into one multiplex assay can cause a significant worsening of the sensitivity. In this study, we propose a simple methodology for the determination of the best arrangement of binding zones, which takes into account the binding constants for immunoreagents. LFIAs of four mycotoxins, namely, aflatoxin B1, deoxynivalenol, T-2 toxin, and ochratoxin A, were integrated into a multiplex test strip. An enzyme-linked immunosorbent assay was applied to determine the equilibrium and kinetic constants of the immunoreactants for each analyte. It was found that the arrangement of binding zones with a descending order of the equilibrium association constants was optimal and provided both lower detection limits and a more uniform coloration. The selected position of the binding zones allowed decreasing the LODs down to 2 and 27 times for ochratoxin A and deoxynivalenol, respectively. The proposed approach can be applied to multiplex LFIAs for different analytes."],"journal":["Micromachines"],"pubmed_title":["Handling Detection Limits of Multiplex Lateral Flow Immunoassay by Choosing the Order of Binding Zones."],"pmcid":["PMC9965744"],"funding_grant_id":["19-14-00370"],"pubmed_authors":["Dzantiev BB","Bartosh AV","Sotnikov DV","Zherdev AV"],"additional_accession":[]},"is_claimable":false,"name":"Handling Detection Limits of Multiplex Lateral Flow Immunoassay by Choosing the Order of Binding Zones.","description":"Changes in the limits of detection (LODs) for a multiplex lateral flow immunoassay (LFIA) caused by different locations of the binding zone on the test strips were studied. Due to the non-equilibrium conditions of the immune reactions in LFIAs, their analytical parameters are susceptible to the binding constants of antigen-antibody reactions and assay duration. Consequently, the integration of several tests into one multiplex assay can cause a significant worsening of the sensitivity. In this study, we propose a simple methodology for the determination of the best arrangement of binding zones, which takes into account the binding constants for immunoreagents. LFIAs of four mycotoxins, namely, aflatoxin B1, deoxynivalenol, T-2 toxin, and ochratoxin A, were integrated into a multiplex test strip. An enzyme-linked immunosorbent assay was applied to determine the equilibrium and kinetic constants of the immunoreactants for each analyte. It was found that the arrangement of binding zones with a descending order of the equilibrium association constants was optimal and provided both lower detection limits and a more uniform coloration. The selected position of the binding zones allowed decreasing the LODs down to 2 and 27 times for ochratoxin A and deoxynivalenol, respectively. The proposed approach can be applied to multiplex LFIAs for different analytes.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-19T07:38:16.57Z","creation":"2025-02-18T23:57:50.102Z"},"accession":"S-EPMC9965744","cross_references":{"pubmed":["36838034"],"doi":["10.3390/mi14020333"]}}