biostudies-literatureWang YAgricultural Science and Technology Innovation Pro- 215 gram (ASTIP) of CAASAgricultural Science and Technology Innovation Program (ASTIP) of CAAS137https://www.ebi.ac.uk/biostudies/studies/S-EPMC9967349biostudies-literatureUnknown15(2)Resistance monitoring in the Asian corn borer, <i>Ostrinia furnacalis</i>, is necessary to accommodate the commercial introduction and stewardship of Bt maize in China. The susceptibility of 56 <i>O. furnacalis</i> field populations, collected between 2015 and 2021 from the corn belt regions of China, to Cry1Ab and Cry1F toxins was determined. Neonate larvae (within 12 h after hatching) were placed on the surface of semi-artificial agar-free diet incorporating a series of concentrations of purified toxins, and mortality was evaluated after 7d. The median lethal concentration (LC₅₀) values of Cry1Ab and Cry1F were 0.05 to 0.37 µg/g (protein/diet) and 0.10 to 1.22 µg/g, respectively. Although interpopulation variation in susceptibility to the toxins was observed, the magnitude of ToxinsSeven Years of Monitoring Susceptibility to Cry1Ab and Cry1F in Asian Corn Borer.PMC9967349NoLiu KZhao WHe KWang YHan SQuan YWang ZChang XWang LfalseSeven Years of Monitoring Susceptibility to Cry1Ab and Cry1F in Asian Corn Borer.Resistance monitoring in the Asian corn borer, <i>Ostrinia furnacalis</i>, is necessary to accommodate the commercial introduction and stewardship of Bt maize in China. The susceptibility of 56 <i>O. furnacalis</i> field populations, collected between 2015 and 2021 from the corn belt regions of China, to Cry1Ab and Cry1F toxins was determined. Neonate larvae (within 12 h after hatching) were placed on the surface of semi-artificial agar-free diet incorporating a series of concentrations of purified toxins, and mortality was evaluated after 7d. The median lethal concentration (LC₅₀) values of Cry1Ab and Cry1F were 0.05 to 0.37 µg/g (protein/diet) and 0.10 to 1.22 µg/g, respectively. Although interpopulation variation in susceptibility to the toxins was observed, the magnitude of 2023-01-01T00:00:00Z2023 Feb2025-04-05T14:19:58.298Z2025-02-18T23:57:55.282ZS-EPMC99673493682845110.3390/toxins15020137