biostudies-literatureShibato JJSPS KAKENHIthe National Research Foundation of Korea (NRF) funded by Ministry of Education, Science, and TechnologyThis work was partly funded by grants from the National Research Foundation of Korea (NRF) funded by Ministry of Education, Science, and Technology (grant no. 2019R1A2C2085868 and 2022R1C1C2003947 provided to STK and CWM).1565https://www.ebi.ac.uk/biostudies/studies/S-EPMC9967486biostudies-literatureUnknown28(4)The biological and psychological importance of hair is recognized worldwide. Molecules that can promote the activation of hair follicle stem cells and the initiation of the growth phase have been subjects of research. Clarifying how hair regeneration is regulated may help to provide hair loss treatments, including cosmetic and even psychological interventions. We examined the hair-growing effects of a cell extract (CE) obtained from cactus <i>Notocactus ottonis</i> by the cold vacuum extraction protocol, by investigating its hair-growing effects, relevant mechanisms, and potential factors therein. Using male C57BL/6 mice, vehicle control (VC: propylene glycol: ethanol: water), MXD (minoxidil, positive control), and <i>N. ottonis</i> CE (N-CE, experimental) were applied topically to the bacMolecules (Basel, Switzerland)Examining the Effect of <i>Notocactus ottonis</i> Cold Vacuum Isolated Plant Cell Extract on Hair Growth in C57BL/6 Mice Using a Combination of Physiological and OMICS Analyses.PMC9967486JP20K20392,20 and JP22H03178JP20K20392,20JP22H031782022R1C1C20039472019R1A2C20858682019R1A2C2085868 and 2022R1C1C2003947Gupta RTakenoya FRakwal RKim STShibato JKimura AShioda SMin CWYamashita MfalseExamining the Effect of <i>Notocactus ottonis</i> Cold Vacuum Isolated Plant Cell Extract on Hair Growth in C57BL/6 Mice Using a Combination of Physiological and OMICS Analyses.The biological and psychological importance of hair is recognized worldwide. Molecules that can promote the activation of hair follicle stem cells and the initiation of the growth phase have been subjects of research. Clarifying how hair regeneration is regulated may help to provide hair loss treatments, including cosmetic and even psychological interventions. We examined the hair-growing effects of a cell extract (CE) obtained from cactus <i>Notocactus ottonis</i> by the cold vacuum extraction protocol, by investigating its hair-growing effects, relevant mechanisms, and potential factors therein. Using male C57BL/6 mice, vehicle control (VC: propylene glycol: ethanol: water), MXD (minoxidil, positive control), and <i>N. ottonis</i> CE (N-CE, experimental) were applied topically to the bac2023-01-01T00:00:00Z2023 Feb2026-04-08T13:32:23.624Z2024-12-03T17:36:44.031ZS-EPMC99674863683855310.3390/molecules28041565