biostudies-literatureLeite ACFundação para a Ciência e Tecnologia4111https://www.ebi.ac.uk/biostudies/studies/S-EPMC9967508biostudies-literatureUnknown24(4)Cdh1p is one of the two substrate adaptor proteins of the anaphase promoting complex/cyclosome (APC/C), a ubiquitin ligase that regulates proteolysis during cell cycle. In this work, using a proteomic approach, we found 135 mitochondrial proteins whose abundance was significantly altered in the <i>cdh1</i>Δ mutant, with 43 up-regulated proteins and 92 down-regulated proteins. The group of significantly up-regulated proteins included subunits of the mitochondrial respiratory chain, enzymes from the tricarboxylic acid cycle and regulators of mitochondrial organization, suggesting a metabolic remodelling towards an increase in mitochondrial respiration. In accordance, mitochondrial oxygen consumption and Cytochrome <i>c</i> oxidase activity increased in Cdh1p-deficient cells. These effects seInternational journal of molecular sciencesThe APC/C Activator Cdh1p Plays a Role in Mitochondrial Metabolic Remodelling in Yeast.PMC9967508SFRH/BD/135921/2018ROTEIRO/0028/2013; LISBOA-01-0145-FEDER-022125IF/00889/2015UIDB/04293/2020Pereira CBarbedo MCosta VLeite ACfalseThe APC/C Activator Cdh1p Plays a Role in Mitochondrial Metabolic Remodelling in Yeast.Cdh1p is one of the two substrate adaptor proteins of the anaphase promoting complex/cyclosome (APC/C), a ubiquitin ligase that regulates proteolysis during cell cycle. In this work, using a proteomic approach, we found 135 mitochondrial proteins whose abundance was significantly altered in the <i>cdh1</i>Δ mutant, with 43 up-regulated proteins and 92 down-regulated proteins. The group of significantly up-regulated proteins included subunits of the mitochondrial respiratory chain, enzymes from the tricarboxylic acid cycle and regulators of mitochondrial organization, suggesting a metabolic remodelling towards an increase in mitochondrial respiration. In accordance, mitochondrial oxygen consumption and Cytochrome <i>c</i> oxidase activity increased in Cdh1p-deficient cells. These effects se2023-01-01T00:00:00Z2023 Feb2025-04-04T21:47:16.258Z2024-11-08T18:11:03.392ZS-EPMC99675083683555510.3390/ijms24044111