{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li L"],"funding":["NIGMS NIH HHS"],"pagination":["2039-2046"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9968529"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["64(11)"],"pubmed_abstract":["In flowering plants (angiosperms), fertilization of the egg cell by one sperm cell produces an embryo, whereas fusion of a second sperm cell with the central cell generates the endosperm. In most angiosperms like Arabidopsis, a pollen grain contains two isomorphic sperm cells required for this double fertilization process. A long-standing unsolved question is whether the two fertilization events have any preference. A tool to address this question is the usage of the cyclin-dependent kinase a1 (cdka;1) mutant pollen, which produces a single sperm-like cell (SLC). Here, we first adopt a complementation-based fluorescence-labeling method to successfully separate and collect cdka;1 mutant pollen containing a single SLC. Single-cell RNA-sequencing analysis revealed that cdka;1 SLCs show a gene"],"journal":["Journal of integrative plant biology"],"pubmed_title":["The egg cell is preferentially fertilized in Arabidopsis double fertilization."],"pmcid":["PMC9968529"],"funding_grant_id":["R35 GM131827"],"pubmed_authors":["Qu LJ","Li J","Gu H","Dresselhaus T","Zhong S","Dong J","Hou S","Xiang W","Zhang L","Wang Y","Li L","Song Z"],"additional_accession":[]},"is_claimable":false,"name":"The egg cell is preferentially fertilized in Arabidopsis double fertilization.","description":"In flowering plants (angiosperms), fertilization of the egg cell by one sperm cell produces an embryo, whereas fusion of a second sperm cell with the central cell generates the endosperm. In most angiosperms like Arabidopsis, a pollen grain contains two isomorphic sperm cells required for this double fertilization process. A long-standing unsolved question is whether the two fertilization events have any preference. A tool to address this question is the usage of the cyclin-dependent kinase a1 (cdka;1) mutant pollen, which produces a single sperm-like cell (SLC). Here, we first adopt a complementation-based fluorescence-labeling method to successfully separate and collect cdka;1 mutant pollen containing a single SLC. Single-cell RNA-sequencing analysis revealed that cdka;1 SLCs show a gene","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-22T09:58:12.649Z","creation":"2025-02-18T23:57:27.972Z"},"accession":"S-EPMC9968529","cross_references":{"pubmed":["36165373"],"doi":["10.1111/jipb.13370"]}}