{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["127(7)"],"submitter":["Howe CP"],"funding":["Science and Technology Facilities Council","University of York"],"pubmed_abstract":["Time-resolved temperature-jump/drop infrared (IR) spectroscopy has been used to measure the impact of stem base sequence on the melting and refolding dynamics of ribonucleic acid (RNA) tetraloops. A series of three 12-nucleotide RNA hairpin sequences were studied, each featuring a UACG tetraloop motif and a double-stranded stem containing four base pairs. In each case, the stem comprised three GC pairs plus a single AU base pair inserted at the closing point of the loop (RNA<sub>loop</sub>), in the middle of the stem (RNA<sub>mid</sub>), or at the stem terminus (RNA<sub>end</sub>). Results from analogous DNA tetraloop (TACG) sequences were also obtained. Inclusion of AU or AT base pairs in the stem leads to faster melting of the stem-loop structure compared to a stem sequence featuring fou"],"journal":["The journal of physical chemistry. B"],"pagination":["1586-1597"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9969394"],"repository":["biostudies-literature"],"pubmed_title":["Sequence-Dependent Melting and Refolding Dynamics of RNA UNCG Tetraloops Using Temperature-Jump/Drop Infrared Spectroscopy."],"pmcid":["PMC9969394"],"pubmed_authors":["Greetham GM","Hunt NT","Parker AW","Howe CP","Procacci B"],"additional_accession":[]},"is_claimable":false,"name":"Sequence-Dependent Melting and Refolding Dynamics of RNA UNCG Tetraloops Using Temperature-Jump/Drop Infrared Spectroscopy.","description":"Time-resolved temperature-jump/drop infrared (IR) spectroscopy has been used to measure the impact of stem base sequence on the melting and refolding dynamics of ribonucleic acid (RNA) tetraloops. A series of three 12-nucleotide RNA hairpin sequences were studied, each featuring a UACG tetraloop motif and a double-stranded stem containing four base pairs. In each case, the stem comprised three GC pairs plus a single AU base pair inserted at the closing point of the loop (RNA<sub>loop</sub>), in the middle of the stem (RNA<sub>mid</sub>), or at the stem terminus (RNA<sub>end</sub>). Results from analogous DNA tetraloop (TACG) sequences were also obtained. Inclusion of AU or AT base pairs in the stem leads to faster melting of the stem-loop structure compared to a stem sequence featuring fou","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-05-29T19:43:14.013Z","creation":"2025-05-29T19:43:14.013Z"},"accession":"S-EPMC9969394","cross_references":{"pubmed":["36787177"],"doi":["10.1021/acs.jpcb.2c08709"]}}