{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Soniat MM"],"funding":["American Cancer Society","Welch Foundation","National Institutes of Health","Cancer Prevention and Research Institute of Texas","UT Austin"],"pagination":["102802"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9971906"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["299(2)"],"pubmed_abstract":["DNA resection-the nucleolytic processing of broken DNA ends-is the first step of homologous recombination. Resection is catalyzed by the resectosome, a multienzyme complex that includes bloom syndrome helicase (BLM), DNA2 or exonuclease 1 nucleases, and additional DNA-binding proteins. Although the molecular players have been known for over a decade, how the individual proteins work together to regulate DNA resection remains unknown. Using single-molecule imaging, we characterized the roles of the MRE11-RAD50-NBS1 complex (MRN) and topoisomerase IIIa (TOP3A)-RMI1/2 during long-range DNA resection. BLM partners with TOP3A-RMI1/2 to form the BTRR (BLM-TOP3A-RMI1/2) complex (or BLM dissolvasome). We determined that TOP3A-RMI1/2 aids BLM in initiating DNA unwinding, and along with MRN, stimula"],"journal":["The Journal of biological chemistry"],"pubmed_title":["The MRN complex and topoisomerase IIIa-RMI1/2 synchronize DNA resection motor proteins."],"pmcid":["PMC9971906"],"funding_grant_id":["CA092584","F-l808","PF-17-169-01-DMC","R1214","GM120554"],"pubmed_authors":["Nguyen G","Kuo HC","Finkelstein IJ","Soniat MM"],"additional_accession":[]},"is_claimable":false,"name":"The MRN complex and topoisomerase IIIa-RMI1/2 synchronize DNA resection motor proteins.","description":"DNA resection-the nucleolytic processing of broken DNA ends-is the first step of homologous recombination. Resection is catalyzed by the resectosome, a multienzyme complex that includes bloom syndrome helicase (BLM), DNA2 or exonuclease 1 nucleases, and additional DNA-binding proteins. Although the molecular players have been known for over a decade, how the individual proteins work together to regulate DNA resection remains unknown. Using single-molecule imaging, we characterized the roles of the MRE11-RAD50-NBS1 complex (MRN) and topoisomerase IIIa (TOP3A)-RMI1/2 during long-range DNA resection. BLM partners with TOP3A-RMI1/2 to form the BTRR (BLM-TOP3A-RMI1/2) complex (or BLM dissolvasome). We determined that TOP3A-RMI1/2 aids BLM in initiating DNA unwinding, and along with MRN, stimula","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-22T02:29:55.318Z","creation":"2025-04-05T20:20:38.889Z"},"accession":"S-EPMC9971906","cross_references":{"pubmed":["36529288"],"doi":["10.1016/j.jbc.2022.102802"]}}