{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ando M"],"funding":["MEXT | Japan Society for the Promotion of Science","Japan Agency for Medical Research and Development"],"pagination":["41-55"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9973402"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["1(1)"],"pubmed_abstract":["T cells with a stem cell memory (T<sub>SCM</sub>) phenotype provide long-term and potent antitumor effects for T-cell transfer therapies. Although various methods for the induction of T<sub>SCM</sub>-like cells <i>in vitro</i> have been reported, few methods generate T<sub>SCM</sub>-like cells from effector/exhausted T cells. We have reported that coculture with the Notch ligand-expressing OP9 stromal cells induces T<sub>SCM</sub>-like (iT<sub>SCM</sub>) cells. Here, we established a feeder-free culture system to improve iT<sub>SCM</sub> cell generation from expanded chimeric antigen receptor (CAR)-expressing T cells; culturing CAR T cells in the presence of IL7, CXCL12, IGF-I, and the Notch ligand, hDLL1. Feeder-free CAR-iT<sub>SCM</sub> cells showed the expression of cell surface markers"],"journal":["Cancer research communications"],"pubmed_title":["Rejuvenating Effector/Exhausted CAR T Cells to Stem Cell Memory-Like CAR T Cells By Resting Them in the Presence of CXCL12 and the NOTCH Ligand."],"pmcid":["PMC9973402"],"funding_grant_id":["21H02719","21H05044","20gm6210012","20gm1110009","21zf0127003h0001","17H06175"],"pubmed_authors":["Shichino S","Yoshimura A","Kondo T","Srirat T","Tomisato W","Ito M","Mise-Omata S","Ando M","Nakagawara K"],"additional_accession":[]},"is_claimable":false,"name":"Rejuvenating Effector/Exhausted CAR T Cells to Stem Cell Memory-Like CAR T Cells By Resting Them in the Presence of CXCL12 and the NOTCH Ligand.","description":"T cells with a stem cell memory (T<sub>SCM</sub>) phenotype provide long-term and potent antitumor effects for T-cell transfer therapies. Although various methods for the induction of T<sub>SCM</sub>-like cells <i>in vitro</i> have been reported, few methods generate T<sub>SCM</sub>-like cells from effector/exhausted T cells. We have reported that coculture with the Notch ligand-expressing OP9 stromal cells induces T<sub>SCM</sub>-like (iT<sub>SCM</sub>) cells. Here, we established a feeder-free culture system to improve iT<sub>SCM</sub> cell generation from expanded chimeric antigen receptor (CAR)-expressing T cells; culturing CAR T cells in the presence of IL7, CXCL12, IGF-I, and the Notch ligand, hDLL1. Feeder-free CAR-iT<sub>SCM</sub> cells showed the expression of cell surface markers","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2026-05-28T17:56:47.62Z","creation":"2024-12-03T15:58:58.327Z"},"accession":"S-EPMC9973402","cross_references":{"pubmed":["36860911"],"doi":["10.1158/2767-9764.CRC-21-0034"]}}