biostudies-literatureLi YNIAID NIH HHSNational Institute of Health68-74https://www.ebi.ac.uk/biostudies/studies/S-EPMC9974537biostudies-literatureUnknown33(1)<h4>Objective</h4>We aimed to develop accurate and user-friendly genetic assays to identify the inherited neutrophil antigen-2 (HNA-2) deficiency in humans.<h4>Background</h4>HNA-2 is one of the most important neutrophil antigens implicated in a number of human disorders. HNA-2 deficiency or HNA-2 null is a common phenotype observed in 3%-5% Americans. HNA-2 null individuals are at risk to produce isoantibodies (or alloantibodies) that play important roles in transfusion-related acute lung injury, immune neutropenia, and bone marrow graft failure. We previously demonstrated that the CD177 coding SNP 787A > T (c.787A > T) is the most important genetic determinant for HNA-2 deficiency. However, reliable genetic assays are not available for routine clinical laboratory application up to now.<hTransfusion medicine (Oxford, England)An accurate genetic assay to identify human neutrophil antigen 2 deficiency.PMC9974537R21 AI149395R21AI149395Wu JLi YSchuller RMfalseAn accurate genetic assay to identify human neutrophil antigen 2 deficiency.<h4>Objective</h4>We aimed to develop accurate and user-friendly genetic assays to identify the inherited neutrophil antigen-2 (HNA-2) deficiency in humans.<h4>Background</h4>HNA-2 is one of the most important neutrophil antigens implicated in a number of human disorders. HNA-2 deficiency or HNA-2 null is a common phenotype observed in 3%-5% Americans. HNA-2 null individuals are at risk to produce isoantibodies (or alloantibodies) that play important roles in transfusion-related acute lung injury, immune neutropenia, and bone marrow graft failure. We previously demonstrated that the CD177 coding SNP 787A > T (c.787A > T) is the most important genetic determinant for HNA-2 deficiency. However, reliable genetic assays are not available for routine clinical laboratory application up to now.<h2023-01-01T00:00:00Z2023 Feb2025-04-26T11:57:18.145Z2025-02-19T04:59:55.583ZS-EPMC99745373630806110.1111/tme.12936