biostudies-literatureUnknown14Stocco ESevere tracheal injuries that cannot be managed by mobilization and end-to-end anastomosis represent an unmet clinical need and an urgent challenge to face in surgical practice; within this scenario, decellularized scaffolds (eventually bioengineered) are currently a tempting option among tissue engineered substitutes. The success of a decellularized trachea is expression of a balanced approach in cells removal while preserving the extracellular matrix (ECM) architecture/mechanical properties. Revising the literature, many Authors report about different methods for acellular tracheal ECMs development; however, only few of them verified the devices effectiveness by an orthotopic implant in animal models of disease. To support translational medicine in this field, here we provide a systematiJournal of tissue engineering20417314231151826https://www.ebi.ac.uk/biostudies/studies/S-EPMC9974632biostudies-literaturePreclinical and clinical orthotopic transplantation of decellularized/engineered tracheal scaffolds: A systematic literature review.PMC9974632De Caro RMacchi VPorzionato AMammana MContran MStocco ERea FParnigotto PPConconi MTBarbon SZambello GfalsePreclinical and clinical orthotopic transplantation of decellularized/engineered tracheal scaffolds: A systematic literature review.Severe tracheal injuries that cannot be managed by mobilization and end-to-end anastomosis represent an unmet clinical need and an urgent challenge to face in surgical practice; within this scenario, decellularized scaffolds (eventually bioengineered) are currently a tempting option among tissue engineered substitutes. The success of a decellularized trachea is expression of a balanced approach in cells removal while preserving the extracellular matrix (ECM) architecture/mechanical properties. Revising the literature, many Authors report about different methods for acellular tracheal ECMs development; however, only few of them verified the devices effectiveness by an orthotopic implant in animal models of disease. To support translational medicine in this field, here we provide a systemati2023-01-01T00:00:00Z2023 Jan-Dec2026-03-17T15:46:09.324Z2025-08-17T03:05:53.725ZS-EPMC99746323687498410.1177/20417314231151826