{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kozhinov AN"],"funding":["Princeton University","European Research Council","Princeton Catalysis Initiative, Princeton University","Biological and Environmental Research","H2020 Future and Emerging Technologies","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["3712-3719"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9974827"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["95(7)"],"pubmed_abstract":["In tandem mass spectrometry (MS2)-based multiplexed quantitative proteomics, the complement reporter ion approaches (TMTc and TMTproC) were developed to eliminate the ratio-compression problem of conventional MS2-level approaches. Resolving all high <i>m</i>/<i>z</i> complement reporter ions (∼6.32 mDa-spaced) requires mass resolution and scan speeds above the performance levels of Orbitrap<sup>TM</sup> instruments. Therefore, complement reporter ion quantification with TMT/TMTpro reagents is currently limited to 5 out of 11 (TMT) or 9 out of 18 (TMTpro) channels (∼1 Da spaced). We first demonstrate that a Fusion<sup>TM</sup> Lumos<sup>TM</sup> Orbitrap can resolve 6.32 mDa-spaced complement reporter ions with standard acquisition modes extended with 3 s transients. We then implemented a s"],"journal":["Analytical chemistry"],"pubmed_title":["Super-Resolution Mass Spectrometry Enables Rapid, Accurate, and Highly Multiplexed Proteomics at the MS2 Level."],"pmcid":["PMC9974827"],"funding_grant_id":["R35 GM128813","R35GM128813","DE-SC0018260","964553","280271"],"pubmed_authors":["Martin WL","Corthesy J","Wuhr M","Johnson A","Kozhinov AN","Tsybin YO","Stadlmeier M","Dayon L","Nagornov KO"],"additional_accession":[]},"is_claimable":false,"name":"Super-Resolution Mass Spectrometry Enables Rapid, Accurate, and Highly Multiplexed Proteomics at the MS2 Level.","description":"In tandem mass spectrometry (MS2)-based multiplexed quantitative proteomics, the complement reporter ion approaches (TMTc and TMTproC) were developed to eliminate the ratio-compression problem of conventional MS2-level approaches. Resolving all high <i>m</i>/<i>z</i> complement reporter ions (∼6.32 mDa-spaced) requires mass resolution and scan speeds above the performance levels of Orbitrap<sup>TM</sup> instruments. Therefore, complement reporter ion quantification with TMT/TMTpro reagents is currently limited to 5 out of 11 (TMT) or 9 out of 18 (TMTpro) channels (∼1 Da spaced). We first demonstrate that a Fusion<sup>TM</sup> Lumos<sup>TM</sup> Orbitrap can resolve 6.32 mDa-spaced complement reporter ions with standard acquisition modes extended with 3 s transients. We then implemented a s","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-03-18T14:13:14.516Z","creation":"2025-04-04T13:15:41.199Z"},"accession":"S-EPMC9974827","cross_references":{"pubmed":["36749928"],"doi":["10.1021/acs.analchem.2c04742"]}}