{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Caza TN"],"funding":["NIDDK NIH HHS","NIMHD NIH HHS","National Institutes of Health"],"pagination":["593-606"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9974866"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["103(3)"],"pubmed_abstract":["Multiple autoantigens have been identified in membranous nephropathy (MN) by tissue-based proteomics. However, antigenic targets of disease are unknown for over 10% of patients with MN and over half of those with membranous lupus nephritis (MLN). Here, we identified multiple new targets in PLA2R-/THSD7A-/EXT-/NELL1-quadruple negative MN biopsies through mass spectrometry of immune complexes recovered from biopsy tissue of patients with MN. Patients with MN negative for these four antigens were identified from Arkana Laboratories case archives. Protein G immunoprecipitation recovered immune complexes from frozen biopsy tissue from 142 quadruple-negative cases and 278 cases of known antigen type, followed by interrogation by mass spectrometry. Potential putative antigens were confirmed throu"],"journal":["Kidney international"],"pubmed_title":["Discovery of seven novel putative antigens in membranous nephropathy and membranous lupus nephritis identified by mass spectrometry."],"pmcid":["PMC9974866"],"funding_grant_id":["MD014110","R43 MD014110","R44 DK130702","1R41DK130702","R41 DK130702"],"pubmed_authors":["Edmondson RD","Larsen CP","Arthur JM","Hassen SI","Kenan DJ","Storey AJ","Herzog C","Caza TN"],"additional_accession":[]},"is_claimable":false,"name":"Discovery of seven novel putative antigens in membranous nephropathy and membranous lupus nephritis identified by mass spectrometry.","description":"Multiple autoantigens have been identified in membranous nephropathy (MN) by tissue-based proteomics. However, antigenic targets of disease are unknown for over 10% of patients with MN and over half of those with membranous lupus nephritis (MLN). Here, we identified multiple new targets in PLA2R-/THSD7A-/EXT-/NELL1-quadruple negative MN biopsies through mass spectrometry of immune complexes recovered from biopsy tissue of patients with MN. Patients with MN negative for these four antigens were identified from Arkana Laboratories case archives. Protein G immunoprecipitation recovered immune complexes from frozen biopsy tissue from 142 quadruple-negative cases and 278 cases of known antigen type, followed by interrogation by mass spectrometry. Potential putative antigens were confirmed throu","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2026-06-01T17:21:41.848Z","creation":"2025-04-04T20:40:11.185Z"},"accession":"S-EPMC9974866","cross_references":{"pubmed":["36638888"],"doi":["10.1016/j.kint.2023.01.001"]}}