{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14"],"submitter":["Xie T"],"funding":["National Natural Science Foundation of China","Agriculture Research System of China"],"pubmed_abstract":["Autophagy plays an important role in host antiviral defense. The avian leukosis virus subgroup J (ALV-J) has been shown to inhibit autophagy while promoting viral replication. The underlying autophagic mechanisms, however, are unknown. Cholesterol 25-hydroxylase (CH25H) is a conserved interferon-stimulated gene, which converts cholesterol to a soluble antiviral factor, 25-hydroxycholesterol (25HC). In this study, we further investigated the autophagic mechanism of CH25H resistance to ALV-J in chicken embryonic fibroblast cell lines (DF1). Our results found that overexpression of <i>CH25H</i> and treatment with 25HC promoted the autophagic markers microtubule-associated protein 1 light chain 3 II (LC3II) and autophagy-related gene 5(ATG5), while decreased autophagy substrate p62/SQSTM1 (p62"],"journal":["Frontiers in immunology"],"pagination":["1093289"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9975585"],"repository":["biostudies-literature"],"pubmed_title":["Chicken CH25H inhibits ALV-J replication by promoting cellular autophagy."],"pmcid":["PMC9975585"],"pubmed_authors":["Xie T","Li X","Feng M","Shi M","Zhang X","Mo G"],"additional_accession":[]},"is_claimable":false,"name":"Chicken CH25H inhibits ALV-J replication by promoting cellular autophagy.","description":"Autophagy plays an important role in host antiviral defense. The avian leukosis virus subgroup J (ALV-J) has been shown to inhibit autophagy while promoting viral replication. The underlying autophagic mechanisms, however, are unknown. Cholesterol 25-hydroxylase (CH25H) is a conserved interferon-stimulated gene, which converts cholesterol to a soluble antiviral factor, 25-hydroxycholesterol (25HC). In this study, we further investigated the autophagic mechanism of CH25H resistance to ALV-J in chicken embryonic fibroblast cell lines (DF1). Our results found that overexpression of <i>CH25H</i> and treatment with 25HC promoted the autophagic markers microtubule-associated protein 1 light chain 3 II (LC3II) and autophagy-related gene 5(ATG5), while decreased autophagy substrate p62/SQSTM1 (p62","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2025-04-19T22:26:38.958Z","creation":"2025-02-19T02:21:22.104Z"},"accession":"S-EPMC9975585","cross_references":{"pubmed":["36875122"],"doi":["10.3389/fimmu.2023.1093289"]}}