biostudies-literatureJiang MChinese Academy of SciencesMinistry of Science and Technology of the People&apos;s Republic of ChinaNational Natural Science Foundation of ChinaChina Postdoctoral Science FoundationNational Key Research and Development Program of China100670https://www.ebi.ac.uk/biostudies/studies/S-EPMC9976449biostudies-literatureUnknown5(4)<h4>Background & aims</h4>Hepatocyte transplantation has emerged as a possible treatment option for end-stage liver disease. However, an important obstacle to therapeutic success is the low level of engraftment and proliferation of transplanted hepatocytes, which do not survive long enough to exert therapeutic effects. Thus, we aimed to explore the mechanisms of hepatocyte proliferation <i>in vivo</i> and find a way to promote the growth of transplanted hepatocytes.<h4>Methods</h4>Hepatocyte transplantation was performed in <i>Fah</i> ^<i>-/-</i> mice to explore the mechanisms of hepatocyte proliferation <i>in vivo</i>. Guided by <i>in vivo</i> regeneration mechanisms, we identified compounds that promote hepatocyte proliferation <i>in vitro</i>. The <i>in vivo</i> effects of theJHEP reports : innovation in hepatologySmall molecule drugs promote repopulation of transplanted hepatocytes by stimulating cell dedifferentiation.PMC997644982121005XDA16010202817300992022YFA1104701XDA16021308320005042018M642117Xie XTang PYuan QJia XWang GJiang MGuo RHe BAi YfalseSmall molecule drugs promote repopulation of transplanted hepatocytes by stimulating cell dedifferentiation.<h4>Background & aims</h4>Hepatocyte transplantation has emerged as a possible treatment option for end-stage liver disease. However, an important obstacle to therapeutic success is the low level of engraftment and proliferation of transplanted hepatocytes, which do not survive long enough to exert therapeutic effects. Thus, we aimed to explore the mechanisms of hepatocyte proliferation <i>in vivo</i> and find a way to promote the growth of transplanted hepatocytes.<h4>Methods</h4>Hepatocyte transplantation was performed in <i>Fah</i> ^<i>-/-</i> mice to explore the mechanisms of hepatocyte proliferation <i>in vivo</i>. Guided by <i>in vivo</i> regeneration mechanisms, we identified compounds that promote hepatocyte proliferation <i>in vitro</i>. The <i>in vivo</i> effects of the2023-01-01T00:00:00Z2023 Apr2025-04-04T20:09:52.255Z2025-02-19T02:21:41.209ZS-EPMC99764493687342010.1016/j.jhepr.2023.100670