biostudies-literatureTheocharidis GNIBIB NIH HHSNIDDK NIH HHS121692https://www.ebi.ac.uk/biostudies/studies/S-EPMC9977170biostudies-literatureUnknown288Diabetic foot ulceration is a devastating diabetic complication with unmet needs. We explored the efficacy of calcium-crosslinked alginate dressings in topically delivering primary macrophages and their secretome to diabetic wounds. The alginate bandages had a microporous structure that enabled even cell loading with prolonged cell survival and egress following wound placement. In vitro experiments showed that we could successfully differentiate and polarize primary murine bone marrow derived monocytes into M0, M1, M2a and M2c defined states with distinct gene expression, surface protein and secretome profiles. The primary macrophages were delivered in the bandages, migrated within the wounds and were still present for as long as 16 days post-injury. In wounds of db/db mice, treatment withBiomaterialsMurine macrophages or their secretome delivered in alginate dressings enhance impaired wound healing in diabetic mice.PMC9977170T32 EB016652DP3 DK108224Niewczas MLee SMoon SVeves ARahmani STheocharidis GMooney DKatopodi XLLi ZWang PKounas KLobao AVlachos ISfalseMurine macrophages or their secretome delivered in alginate dressings enhance impaired wound healing in diabetic mice.Diabetic foot ulceration is a devastating diabetic complication with unmet needs. We explored the efficacy of calcium-crosslinked alginate dressings in topically delivering primary macrophages and their secretome to diabetic wounds. The alginate bandages had a microporous structure that enabled even cell loading with prolonged cell survival and egress following wound placement. In vitro experiments showed that we could successfully differentiate and polarize primary murine bone marrow derived monocytes into M0, M1, M2a and M2c defined states with distinct gene expression, surface protein and secretome profiles. The primary macrophages were delivered in the bandages, migrated within the wounds and were still present for as long as 16 days post-injury. In wounds of db/db mice, treatment with2022-01-01T00:00:00Z2022 Sep2025-04-04T20:04:25.333Z2025-04-04T20:04:25.333ZS-EPMC99771703593452010.1016/j.biomaterials.2022.121692