biostudies-literatureTong XNational Natural Science Foundation of ChinaSpecial Project for Research and Development in Key areas of Guangdong ProvinceChangsha Science and Technology Project100-112https://www.ebi.ac.uk/biostudies/studies/S-EPMC9984782biostudies-literatureUnknown39<h4>Background</h4>The inadequate regeneration of natural tissue (mainly fibrocartilage) between tendon and bone during rotator cuff (RC) repair results in an unsatisfactory quality of RC healing. Cell-free therapy based on stem cell exosomes is a safer and more promising approach for tissue regeneration. Here, we investigated the effect of exosomes from human urine-derived stem cells (USCs) and their subpopulations (CD133⁺USCs) on RC healing.<h4>Methods</h4>USCs were isolated from urine and sorted by flow cytometry to obtain CD133⁺ urine-derived stem cells (CD133⁺ USCs). Urine-derived stem cell exosomes (USC-Exos) and CD133⁺ urine-derived stem cell exosomes (CD133⁺ USC-Exos) were subsequently isolated from the cell supernatant and ideJournal of orthopaedic translationExosomes from CD133⁺ human urine-derived stem cells combined adhesive hydrogel facilitate rotator cuff healing by mediating bone marrow mesenchymal stem cells.PMC9984782kh210201581730068819743382020SK2077Deng CZhang TXun JSun DXu DTong XXu YfalseExosomes from CD133⁺ human urine-derived stem cells combined adhesive hydrogel facilitate rotator cuff healing by mediating bone marrow mesenchymal stem cells.<h4>Background</h4>The inadequate regeneration of natural tissue (mainly fibrocartilage) between tendon and bone during rotator cuff (RC) repair results in an unsatisfactory quality of RC healing. Cell-free therapy based on stem cell exosomes is a safer and more promising approach for tissue regeneration. Here, we investigated the effect of exosomes from human urine-derived stem cells (USCs) and their subpopulations (CD133⁺USCs) on RC healing.<h4>Methods</h4>USCs were isolated from urine and sorted by flow cytometry to obtain CD133⁺ urine-derived stem cells (CD133⁺ USCs). Urine-derived stem cell exosomes (USC-Exos) and CD133⁺ urine-derived stem cell exosomes (CD133⁺ USC-Exos) were subsequently isolated from the cell supernatant and ide2023-01-01T00:00:00Z2023 Mar2025-04-04T11:35:48.035Z2025-04-04T11:35:48.035ZS-EPMC99847823687979410.1016/j.jot.2023.02.002