{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Spitzberg JD"],"funding":["NCI NIH HHS","Center for Strategic Scientific Initiatives, National Cancer Institute"],"pagination":["1239"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9985597"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Exosomes and extracellular vesicles (EV) are increasingly being explored as circulating biomarkers, but their heterogenous composition will likely mandate the development of multiplexed EV technologies. Iteratively multiplexed analyses of near single EVs have been challenging to implement beyond a few colors during spectral sensing. Here we developed a multiplexed analysis of EV technique (MASEV) to interrogate thousands of individual EVs during 5 cycles of multi-channel fluorescence staining for 15 EV biomarkers. Contrary to the common belief, we show that: several markers proposed to be ubiquitous are less prevalent than believed; multiple biomarkers concur in single vesicles but only in small fractions; affinity purification can lead to loss of rare EV subtypes; and deep profiling allow"],"journal":["Nature communications"],"pubmed_title":["Multiplexed analysis of EV reveals specific biomarker composition with diagnostic impact."],"pmcid":["PMC9985597"],"funding_grant_id":["T32 CA079443","R01 CA237332","P01CA069246","R21CA236561","T32CA079443","P01 CA069246"],"pubmed_authors":["Peterson HM","Weissleder R","Ferguson S","Spitzberg JD","Yang KS","Carlson JCT"],"additional_accession":[]},"is_claimable":false,"name":"Multiplexed analysis of EV reveals specific biomarker composition with diagnostic impact.","description":"Exosomes and extracellular vesicles (EV) are increasingly being explored as circulating biomarkers, but their heterogenous composition will likely mandate the development of multiplexed EV technologies. Iteratively multiplexed analyses of near single EVs have been challenging to implement beyond a few colors during spectral sensing. Here we developed a multiplexed analysis of EV technique (MASEV) to interrogate thousands of individual EVs during 5 cycles of multi-channel fluorescence staining for 15 EV biomarkers. Contrary to the common belief, we show that: several markers proposed to be ubiquitous are less prevalent than believed; multiple biomarkers concur in single vesicles but only in small fractions; affinity purification can lead to loss of rare EV subtypes; and deep profiling allow","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2026-05-29T02:41:40.653Z","creation":"2024-12-03T21:24:39.728Z"},"accession":"S-EPMC9985597","cross_references":{"pubmed":["36870999"],"doi":["10.1038/s41467-023-36932-z"]}}