biostudies-literatureUnknown10Shang MHNational Natural Science Foundation of ChinaPhycocyanin and allophycocyanin are important active substances in <i>Arthrospira platensis</i>, because of their fluorescent characteristic and antioxidant capacity. In order to solve the problem of insufficient production and inconvenient modification of natural protein, recombinant expression was performed and the fluorescence activity and antioxidant activity was analyzed to meet the demand for phycocyanin and allophycocyanin. A total of seven recombinant strains were constructed in this study, including individual phycocyanin or allophycocyanin, co-expression of phycocyanin-allophycocyanin, and their co-expression with chromophore, and the expression strain for individual chromophore. Different molecular weights of phycocyanin and allophycocyanin were detected in the recombinant straiFrontiers in nutrition1127422https://www.ebi.ac.uk/biostudies/studies/S-EPMC9987159biostudies-literatureFluorescence and antioxidant activity of heterologous expression of phycocyanin and allophycocyanin from <i>Arthrospira platensis</i>.PMC9987159Xu XTZang XNSun JFShang MHBi YfalseFluorescence and antioxidant activity of heterologous expression of phycocyanin and allophycocyanin from <i>Arthrospira platensis</i>.Phycocyanin and allophycocyanin are important active substances in <i>Arthrospira platensis</i>, because of their fluorescent characteristic and antioxidant capacity. In order to solve the problem of insufficient production and inconvenient modification of natural protein, recombinant expression was performed and the fluorescence activity and antioxidant activity was analyzed to meet the demand for phycocyanin and allophycocyanin. A total of seven recombinant strains were constructed in this study, including individual phycocyanin or allophycocyanin, co-expression of phycocyanin-allophycocyanin, and their co-expression with chromophore, and the expression strain for individual chromophore. Different molecular weights of phycocyanin and allophycocyanin were detected in the recombinant strai2023-01-01T00:00:00Z20232026-04-08T12:33:44.328Z2025-04-07T03:19:52.154ZS-EPMC99871593689116210.3389/fnut.2023.1127422