biostudies-literatureUnknown29(4)Kaushal N<h4>Introduction</h4>Crimean-Congo haemorrhagic fever virus (CCHFV) has tripartite RNA genome and is endemic in various countries of Asia, Africa and Europe.<h4>Method</h4>The present study is focused on mutation profiling of CCHFV L segment and phylogenetic clustering of protein dataset into six CCHFV genotypes.<h4>Results</h4>Phylogenetic tree rooted with NCBI reference sequence (YP_325663.1) indicated less divergence from genotype III and the sequences belonging to same genotypes have shown less divergence among each other. Mutation frequency at 729 mutated positions was calculated and 563, 49, 33, 46 and 38 amino acid positions were found to be mutated at mutation frequency intervals of 0-0.2, 0.21-0.4, 0.41-0.6, 0.61-0.8 and 0.81-1.0 respectively. Thirty-eight highly frequent mutationJournal of molecular modeling88https://www.ebi.ac.uk/biostudies/studies/S-EPMC9987378biostudies-literatureMutational analysis of catalytic site domain of CCHFV L RNA segment.PMC9987378Baranwal MKaushal NfalseMutational analysis of catalytic site domain of CCHFV L RNA segment.<h4>Introduction</h4>Crimean-Congo haemorrhagic fever virus (CCHFV) has tripartite RNA genome and is endemic in various countries of Asia, Africa and Europe.<h4>Method</h4>The present study is focused on mutation profiling of CCHFV L segment and phylogenetic clustering of protein dataset into six CCHFV genotypes.<h4>Results</h4>Phylogenetic tree rooted with NCBI reference sequence (YP_325663.1) indicated less divergence from genotype III and the sequences belonging to same genotypes have shown less divergence among each other. Mutation frequency at 729 mutated positions was calculated and 563, 49, 33, 46 and 38 amino acid positions were found to be mutated at mutation frequency intervals of 0-0.2, 0.21-0.4, 0.41-0.6, 0.61-0.8 and 0.81-1.0 respectively. Thirty-eight highly frequent mutation2023-01-01T00:00:00Z2023 Mar2026-07-14T19:07:39.323Z2025-02-18T23:44:39.617ZS-EPMC99873783687725810.1007/s00894-023-05487-7