{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(1)"],"submitter":["Mohamed BM"],"pubmed_abstract":["Circulating tumour cells (CTCs) are a critical intermediate step in the process of cancer metastasis. The reliability of CTC isolation/purification has limited both the potential to report on metastatic progression and the development of CTCs as targets for therapeutic intervention. Here we report a new methodology, which optimises the culture conditions for CTCs using primary cancer cells as a model system. We exploited the known biology that CTCs thrive in hypoxic conditions, with their survival and proliferation being reliant on the activation of hypoxia-inducible factor 1 alpha (HIF-1α). We isolated epithelial-like and quasi-mesenchymal CTC phenotypes from the blood of a cancer patient and successfully cultured these cells for more than 8 weeks. The presence of CTC clusters was require"],"journal":["Scientific reports"],"pagination":["3704"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9988863"],"repository":["biostudies-literature"],"pubmed_title":["Ex vivo expansion of circulating tumour cells (CTCs)."],"pmcid":["PMC9988863"],"pubmed_authors":["O'Leary JJ","Selemidis S","Kelly T","Bates M","Gallagher M","Heffernan S","Saadeh FA","Brooks DA","Gleeson N","Mohamed BM","Martin C","Spillane CD","Kennedy J","Norris L","O'Toole S","Brooks RD","Ward MP"],"additional_accession":[]},"is_claimable":false,"name":"Ex vivo expansion of circulating tumour cells (CTCs).","description":"Circulating tumour cells (CTCs) are a critical intermediate step in the process of cancer metastasis. The reliability of CTC isolation/purification has limited both the potential to report on metastatic progression and the development of CTCs as targets for therapeutic intervention. Here we report a new methodology, which optimises the culture conditions for CTCs using primary cancer cells as a model system. We exploited the known biology that CTCs thrive in hypoxic conditions, with their survival and proliferation being reliant on the activation of hypoxia-inducible factor 1 alpha (HIF-1α). We isolated epithelial-like and quasi-mesenchymal CTC phenotypes from the blood of a cancer patient and successfully cultured these cells for more than 8 weeks. The presence of CTC clusters was require","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2025-04-21T19:47:13.818Z","creation":"2025-04-05T17:43:24.968Z"},"accession":"S-EPMC9988863","cross_references":{"pubmed":["36879003"],"doi":["10.1038/s41598-023-30733-6"]}}