{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Davidson LA"],"funding":["NICHD NIH HHS"],"pagination":["Pdb.prot097386"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9989777"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2022(11)"],"pubmed_abstract":["This protocol summarizes preparation of the dorsal marginal zone sandwich explant (a.k.a. the \"Keller sandwich\") and the dorsal isolate from <i>Xenopus</i> embryos. The Keller sandwich is assembled from two early gastrula stage dorsal marginal zone (DMZ) explants. DMZ explants isolated before involution maintain planar patterning processes and block radial signals that might be exchanged between pre- and postinvolution tissues. DMZ explants isolated later in gastrulation, but subsequently opened and flattened may have both planar and radial patterning. The epithelial margins of DMZ explants in Keller sandwiches heal and basal contacts form between the deep layers of the two DMZ explants. The dorsal isolate is dissected from mid- to late-gastrula stage embryos after involution and archenter"],"journal":["Cold Spring Harbor protocols"],"pubmed_title":["Microsurgical Methods to Make the Keller Sandwich Explant and the Dorsal Isolate."],"pmcid":["PMC9989777"],"funding_grant_id":["R01 HD044750","R21 HD106629"],"pubmed_authors":["Davidson LA"],"additional_accession":[]},"is_claimable":false,"name":"Microsurgical Methods to Make the Keller Sandwich Explant and the Dorsal Isolate.","description":"This protocol summarizes preparation of the dorsal marginal zone sandwich explant (a.k.a. the \"Keller sandwich\") and the dorsal isolate from <i>Xenopus</i> embryos. The Keller sandwich is assembled from two early gastrula stage dorsal marginal zone (DMZ) explants. DMZ explants isolated before involution maintain planar patterning processes and block radial signals that might be exchanged between pre- and postinvolution tissues. DMZ explants isolated later in gastrulation, but subsequently opened and flattened may have both planar and radial patterning. The epithelial margins of DMZ explants in Keller sandwiches heal and basal contacts form between the deep layers of the two DMZ explants. The dorsal isolate is dissected from mid- to late-gastrula stage embryos after involution and archenter","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-26T22:58:02.08Z","creation":"2025-04-06T17:23:31.859Z"},"accession":"S-EPMC9989777","cross_references":{"pubmed":["35577523"],"doi":["10.1101/pdb.prot097386"]}}