biostudies-literatureField DHUniversity of LeedsLeverhulme TrustRoyal SocietyWellcome TrustEngineering and Physical Sciences Research Council216-222https://www.ebi.ac.uk/biostudies/studies/S-EPMC9994102biostudies-literatureUnknown4(3)Fluorescent ligands for G-protein coupled receptors (GPCRs) are valuable tools for studying the expression, pharmacology and modulation of these therapeutically important proteins in living cells. Here we report a fluorescent photoaffinity probe for Formyl peptide receptor 1 (FPR1), a critical component of the innate immune response to bacterial infection and a promising target in inflammatory diseases. We demonstrate that the probe binds and covalently crosslinks to FPR1 with good specificity at nanomolar concentrations in living cells and is a useful tool for visualisation and characterisation of this receptor.RSC chemical biologyA fluorescent photoaffinity probe for formyl peptide receptor 1 labelling in living cells.PMC9994102RG1609141930246WT104918MARPG-2018-030Wright MHField DHWhite JSWarriner SLfalseA fluorescent photoaffinity probe for formyl peptide receptor 1 labelling in living cells.Fluorescent ligands for G-protein coupled receptors (GPCRs) are valuable tools for studying the expression, pharmacology and modulation of these therapeutically important proteins in living cells. Here we report a fluorescent photoaffinity probe for Formyl peptide receptor 1 (FPR1), a critical component of the innate immune response to bacterial infection and a promising target in inflammatory diseases. We demonstrate that the probe binds and covalently crosslinks to FPR1 with good specificity at nanomolar concentrations in living cells and is a useful tool for visualisation and characterisation of this receptor.2023-01-01T00:00:00Z2023 Mar2026-05-28T22:45:22.667Z2025-04-04T20:21:47.736ZS-EPMC99941023690870110.1039/d2cb00199c