{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Mehri M"],"pubmed_abstract":["<h4>Introduction</h4>The microenvironment of solid tumors such as breast cancer is heterogeneous and complex, containing different types of cell, namely, cancer stem cells and immune cells. We previously reported the immunoregulatory behavior of the human immune cell in a solid tumor microenvironment-like culture under serum starvation stress for 96 h. Here, we examined the effect of this culture-derived solution on breast cancer development in rats.<h4>Method</h4>Ninety-six-hour starved PBMCs supernatant (96 h-SPS) was collected after culturing human PBMCs for 96 h under serum starvation condition. Breast cancer stem cells, LA7 cell line, was used for <i>in vitro</i> study by analyzing gene expression status and performing cytotoxicity, proliferation, scratch wound healing assays, followe"],"journal":["Frontiers in immunology"],"pagination":["1025933"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9996193"],"repository":["biostudies-literature"],"pubmed_title":["Ninety-six-hour starved peripheral blood mononuclear cell supernatant inhibited LA7 breast cancer stem cells induced tumor <i>via</i> reduction in angiogenesis and alternations in <i>Gch1</i> and <i>Spr</i> expressions."],"pmcid":["PMC9996193"],"pubmed_authors":["Kalali B","Mostafazadeh A","Pourbagher R","Khorasani HR","Rahimi SM","Fattahi S","Gheitasi R","Hossein-Nattaj H","Akhavan-Niaki H","Nayeri K","Ranaee M","Sabour D","Mehri M"],"additional_accession":[]},"is_claimable":false,"name":"Ninety-six-hour starved peripheral blood mononuclear cell supernatant inhibited LA7 breast cancer stem cells induced tumor <i>via</i> reduction in angiogenesis and alternations in <i>Gch1</i> and <i>Spr</i> expressions.","description":"<h4>Introduction</h4>The microenvironment of solid tumors such as breast cancer is heterogeneous and complex, containing different types of cell, namely, cancer stem cells and immune cells. We previously reported the immunoregulatory behavior of the human immune cell in a solid tumor microenvironment-like culture under serum starvation stress for 96 h. Here, we examined the effect of this culture-derived solution on breast cancer development in rats.<h4>Method</h4>Ninety-six-hour starved PBMCs supernatant (96 h-SPS) was collected after culturing human PBMCs for 96 h under serum starvation condition. Breast cancer stem cells, LA7 cell line, was used for <i>in vitro</i> study by analyzing gene expression status and performing cytotoxicity, proliferation, scratch wound healing assays, followe","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-29T01:21:34.073Z","creation":"2024-11-13T20:22:43.909Z"},"accession":"S-EPMC9996193","cross_references":{"pubmed":["36908807"],"doi":["10.3389/fimmu.2022.1025933"]}}