{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang J"],"funding":["Beijing Municipal Natural Science Foundation","CAMS Innovation Fund for Medical Sciences","National Natural Science Foundation of China","Independent Issue of State Key Laboratory of Molecular Oncology","Open Issue of State Key Laboratory of Molecular Oncology","National Key Research and Development Program of China"],"pagination":["50"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9996991"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Background
Breast cancer (BC) metastasis is the leading cause of poor prognosis and therapeutic failure. However, the mechanisms underlying cancer metastasis are far from clear.Methods
We screened candidate genes related to metastasis through genome-wide CRISPR screening and high-throughput sequencing of patients with metastatic BC, followed by a panel of metastatic model assays. The effects of tetratricopeptide repeat domain 17 (TTC17) on migration, invasion, and colony formation ability together with the responses to anticancer drugs were investigated in vitro and in vivo. The mechanism mediated by TTC17 was determined by RNA sequencing, Western blotting, immunohistochemistry, and immunofluorescence. The clinical significance of TTC17 was evaluated using BC tissue samples"],"journal":["Cell & bioscience"],"pubmed_title":["Loss of TTC17 promotes breast cancer metastasis through RAP1/CDC42 signaling and sensitizes it to rapamycin and paclitaxel."],"pmcid":["PMC9996991"],"funding_grant_id":["81872280","7222145","82073094","SKL-2021-16","2022YFE0103600","SKL-KF-2021-16","2021YFF1201300","82230058","2021-I2M-014"],"pubmed_authors":["Qian H","Li C","Zhang J","Guo F","Sun F","Wang Y","Zhou Y","Wang J","Zhang B","Ma F"],"additional_accession":[]},"is_claimable":false,"name":"Loss of TTC17 promotes breast cancer metastasis through RAP1/CDC42 signaling and sensitizes it to rapamycin and paclitaxel.","description":"Background
Breast cancer (BC) metastasis is the leading cause of poor prognosis and therapeutic failure. However, the mechanisms underlying cancer metastasis are far from clear.Methods
We screened candidate genes related to metastasis through genome-wide CRISPR screening and high-throughput sequencing of patients with metastatic BC, followed by a panel of metastatic model assays. The effects of tetratricopeptide repeat domain 17 (TTC17) on migration, invasion, and colony formation ability together with the responses to anticancer drugs were investigated in vitro and in vivo. The mechanism mediated by TTC17 was determined by RNA sequencing, Western blotting, immunohistochemistry, and immunofluorescence. The clinical significance of TTC17 was evaluated using BC tissue samples","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2025-04-21T22:48:53.606Z","creation":"2024-11-15T10:07:32.418Z"},"accession":"S-EPMC9996991","cross_references":{"pubmed":["36895029"],"doi":["10.1186/s13578-023-01004-8"]}}