biostudies-literatureMa WNatural Science Foundation of Shandong ProvinceCultivate Fund from The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan HospitalClinical & Medical Science and Technology Innovation Program of JinanCultivate Fund from The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan HospitalClinical & Medical Science and Technology Innovation Program of Jinan471-480https://www.ebi.ac.uk/biostudies/studies/S-EPMC9998317biostudies-literatureUnknown42(4)The widespread of different NDM variants in clinical Enterobacterales isolates poses a serious public health concern, which requires continuous monitoring. In this study, three E. coli strains carrying two novel bla<sub>NDM</sub> variants of bla<sub>NDM-36, -37</sub> were identified from a patient with refractory urinary tract infection (UTI) in China. We conducted antimicrobial susceptibility testing (AST), enzyme kinetics analysis, conjugation experiment, whole-genome sequencing (WGS), and bioinformatics analysis to characterize the bla<sub>NDM-36, -37</sub> enzymes and their carrying strains. The bla<sub>NDM-36, -37</sub> harboring E. coli isolates belonged to ST227, O9:H10 serotype and exhibited intermediate or resistance to all β-lactams tested except aztreonam and aztreonam/avibactamEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical MicrobiologyGenetic and enzymatic characterization of two novel bla<sub>NDM-36, -37</sub> variants in Escherichia coli strains.PMC9998317QYPY2022NSFC0802ZR2021MH078202134040Cheng FDong XChen LHao MCui XZhu BShi XMa WLi XNiu SWang QWang WMa JWang YfalseGenetic and enzymatic characterization of two novel bla<sub>NDM-36, -37</sub> variants in Escherichia coli strains.The widespread of different NDM variants in clinical Enterobacterales isolates poses a serious public health concern, which requires continuous monitoring. In this study, three E. coli strains carrying two novel bla<sub>NDM</sub> variants of bla<sub>NDM-36, -37</sub> were identified from a patient with refractory urinary tract infection (UTI) in China. We conducted antimicrobial susceptibility testing (AST), enzyme kinetics analysis, conjugation experiment, whole-genome sequencing (WGS), and bioinformatics analysis to characterize the bla<sub>NDM-36, -37</sub> enzymes and their carrying strains. The bla<sub>NDM-36, -37</sub> harboring E. coli isolates belonged to ST227, O9:H10 serotype and exhibited intermediate or resistance to all β-lactams tested except aztreonam and aztreonam/avibactam2023-01-01T00:00:00Z2023 Apr2025-05-29T20:46:54.17Z2024-11-07T08:46:02.831ZS-EPMC99983173681072610.1007/s10096-023-04576-y