{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jansen KI"],"funding":["European Molecular Biology Organization","European Research Council","Dutch Research Council (NWO)"],"pagination":["e202106105"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9998657"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["222(5)"],"pubmed_abstract":["The microtubule (MT) cytoskeleton underlies processes such as intracellular transport and cell division. Immunolabeling for posttranslational modifications of tubulin has revealed the presence of different MT subsets, which are believed to differ in stability and function. Whereas dynamic MTs can readily be studied using live-cell plus-end markers, the dynamics of stable MTs have remained obscure due to a lack of tools to directly visualize these MTs in living cells. Here, we present StableMARK (Stable Microtubule-Associated Rigor-Kinesin), a live-cell marker to visualize stable MTs with high spatiotemporal resolution. We demonstrate that a rigor mutant of Kinesin-1 selectively binds to stable MTs without affecting MT organization and organelle transport. These MTs are long-lived, undergo "],"journal":["The Journal of cell biology"],"pubmed_title":["A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle."],"pmcid":["PMC9998657"],"funding_grant_id":["022.006.001","819219","ALTF 407-2017"],"pubmed_authors":["Burute M","Kapitein LC","Iwanski MK","Jansen KI"],"additional_accession":[]},"is_claimable":false,"name":"A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle.","description":"The microtubule (MT) cytoskeleton underlies processes such as intracellular transport and cell division. Immunolabeling for posttranslational modifications of tubulin has revealed the presence of different MT subsets, which are believed to differ in stability and function. Whereas dynamic MTs can readily be studied using live-cell plus-end markers, the dynamics of stable MTs have remained obscure due to a lack of tools to directly visualize these MTs in living cells. Here, we present StableMARK (Stable Microtubule-Associated Rigor-Kinesin), a live-cell marker to visualize stable MTs with high spatiotemporal resolution. We demonstrate that a rigor mutant of Kinesin-1 selectively binds to stable MTs without affecting MT organization and organelle transport. These MTs are long-lived, undergo ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2026-03-17T15:22:22.402Z","creation":"2025-04-19T02:47:42.623Z"},"accession":"S-EPMC9998657","cross_references":{"pubmed":["36880745"],"doi":["10.1083/jcb.202106105"]}}