{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Casey LM"],"funding":["NIBIB NIH HHS","NIDDK NIH HHS","NIAID NIH HHS","NCI NIH HHS","National Institutes of Health"],"pagination":["284-296"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9999438"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["120(1)"],"pubmed_abstract":["Immune-mediated hypersensitivities such as autoimmunity, allergy, and allogeneic graft rejection are treated with therapeutics that suppress the immune system, and the lack of specificity is associated with significant side effects. The delivery of disease-relevant antigens (Ags) by carrier systems such as poly(lactide-co-glycolide) nanoparticles (PLG-Ag) and carbodiimide (ECDI)-fixed splenocytes (SP-Ag) has demonstrated Ag-specific tolerance induction in model systems of these diseases. Despite therapeutic outcomes by both platforms, tolerance is conferred with different efficacy. This investigation evaluated Ag loading and total particle dose of PLG-Ag on Ag presentation in a coculture system of dendritic cells (DCs) and Ag-restricted T cells, with SP-Ag employed as a control. CD25 expre"],"journal":["Biotechnology and bioengineering"],"pubmed_title":["Nanoparticle dose and antigen loading attenuate antigen-specific T-cell responses."],"pmcid":["PMC9999438"],"funding_grant_id":["P30 CA046592","P30 DK020572","R01 AI028076","K01 EB028877"],"pubmed_authors":["Rad L","Decker JT","Rose JA","Podojil JR","Casey LM","Pearson RM","Miller SD","Hughes KR","Shea LD"],"additional_accession":[]},"is_claimable":false,"name":"Nanoparticle dose and antigen loading attenuate antigen-specific T-cell responses.","description":"Immune-mediated hypersensitivities such as autoimmunity, allergy, and allogeneic graft rejection are treated with therapeutics that suppress the immune system, and the lack of specificity is associated with significant side effects. The delivery of disease-relevant antigens (Ags) by carrier systems such as poly(lactide-co-glycolide) nanoparticles (PLG-Ag) and carbodiimide (ECDI)-fixed splenocytes (SP-Ag) has demonstrated Ag-specific tolerance induction in model systems of these diseases. Despite therapeutic outcomes by both platforms, tolerance is conferred with different efficacy. This investigation evaluated Ag loading and total particle dose of PLG-Ag on Ag presentation in a coculture system of dendritic cells (DCs) and Ag-restricted T cells, with SP-Ag employed as a control. CD25 expre","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-04T00:35:01.948Z","creation":"2025-04-04T00:35:01.948Z"},"accession":"S-EPMC9999438","cross_references":{"pubmed":["36221192"],"doi":["10.1002/bit.28252"]}}