biostudies-otherUnknown114Tjerko KammingaBiotechnology and bioengineering2339-2347Mycoplasma hyopneumoniaehttps://www.ebi.ac.uk/biostudies/studies/MODEL1704250001biostudies-other28600895Tjerko KammingafalseKamminga2017 - Metabolic model of Mycoplasma hyopneumoniae growth<notes xmlns="http://www.sbml.org/sbml/level2"> <body xmlns="http://www.w3.org/1999/xhtml"> <div class="dc:title">Kamminga2017 - Metabolic model of Mycoplasma hyopneumoniae growth</div> <div class="dc:bibliographicCitation"> <p>This model is described in the article:</p> <div class="bibo:title"> <a href="http://identifiers.org/pubmed/28600895" title="Access to this publication">Metabolic modeling of energy balances in Mycoplasma hyopneumoniae shows that pyruvate addition increases growth rate.</a> </div> <div class="bibo:authorList">Kamminga T, Slagman SJ, Bijlsma JJE, Martins Dos Santos VAP, Suarez-Diez M, Schaap PJ.</div> <div class="bibo:Journal">Biotechnol. Bioeng. 2017 Jun; :</div> <p>Abstract:</p> <div class="bibo:abstract"> <p>Mycoplasma hyopneumoniae is cultured on large-scale to produce antigen for inactivated whole-cell vaccines against respiratory disease in pigs. However, the fastidious nutrient requirements of this minimal bacterium and the low growth rate make it challenging to reach sufficient biomass yield for antigen production. In this study, we sequenced the genome of M. hyopneumoniae strain 11 and constructed a high quality constraint-based genome-scale metabolic model of 284 chemical reactions and 298 metabolites. We validated the model with time-series data of duplicate fermentation cultures to aim for an integrated model describing the dynamic profiles measured in fermentations. The model predicted that 84% of cellular energy in a standard M. hyopneumoniae cultivation was used for non-growth associated maintenance and only 16% of cellular energy was used for growth and growth associated maintenance. Following a cycle of model-driven experimentation in dedicated fermentation experiments, we were able to increase the fraction of cellular energy used for growth through pyruvate addition to the medium. This increase in turn led to an increase in growth rate and a 2.3 times increase in the total biomass concentration reached after 3-4 days of fermentation, enhancing the productivity of the overall process. The model presented provides a solid basis to understand and further improve M. hyopneumoniae fermentation processes. Biotechnol. Bioeng. 2017;9999: 1-9. © 2017 Wiley Periodicals, Inc.</p> </div> </div> <div class="dc:publisher"> <p>This model is hosted on <a href="http://www.ebi.ac.uk/biomodels/">BioModels Database</a> and identified by: <a href="http://identifiers.org/biomodels.db/MODEL1704250001">MODEL1704250001</a>.</p> <p>To cite BioModels Database, please use: <a href="http://identifiers.org/pubmed/20587024" title="Latest BioModels Database publication">BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models</a>.</p> </div> <div class="dc:license"> <p>To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to <a href="http://creativecommons.org/publicdomain/zero/1.0/" title="Access to: CC0 1.0 Universal (CC0 1.0), Public Domain Dedication">CC0 Public Domain Dedication</a> for more information.</p> </div></body></notes>2017-04-25T00:00:00Z2025-07-14T17:54:40.495Z2025-03-30T22:40:10.971ZMODEL1704250001SBO:0000624286008952099null