biostudies-otherUnknown8Lucian SmithScientific reports2696Homo sapienshttps://www.ebi.ac.uk/biostudies/studies/MODEL2204290002biostudies-other10.1038/s41598-018-20348-7Lucian SmithMikal DaoufalseJeon2018 - Enzyme clustering in Glucose metabolismMiji Jeon, Hye-Won Kang & Songon An. A Mathematical Model for Enzyme Clustering in Glucose Metabolism. Scientific Reports 8, 1 (2018).<p></p><p></p>We have recently demonstrated that the rate-limiting enzymes in human glucose metabolism organize into cytoplasmic clusters to form a multienzyme complex, the glucosome, in at least three different sizes. Quantitative high-content imaging data support a hypothesis that the glucosome clusters regulate the direction of glucose flux between energy metabolism and building block biosynthesis in a cluster size-dependent manner. However, direct measurement of their functional contributions to cellular metabolism at subcellular levels has remained challenging. In this work, we develop a mathematical model using a system of ordinary differential equations, in which the association of the rate-limiting enzymes into multienzyme complexes is included as an essential element. We then demonstrate that our mathematical model provides a quantitative principle to simulate glucose flux at both subcellular and population levels in human cancer cells. Lastly, we use the model to simulate 2-deoxyglucose-mediated alteration of glucose flux in a population level based on subcellular high-content imaging data. Collectively, we introduce a new mathematical model for human glucose metabolism, which promotes our understanding of functional roles of differently sized multienzyme complexes in both single-cell and population levels.2022-04-29T00:00:00Z2025-07-15T09:43:21.173Z2025-03-29T22:32:12.749ZMODEL2204290002SBO:0000330SBO:0000341SBO:0000394SBO:0000217SBO:0000180SBO:0000526SBO:0000009BIOMD0000001055R-HSA-70475R-HSA-70471R-HSA-71496C28597C16981NCIT:C21034CHEBI:30744CHEBI:78697CHEBI:17234CHEBI:25212CHEBI:15361CHEBI:61304CHEBI:18021CHEBI:64297CHEBI:78682MAMO_0000046GO:0004634GO:0004611GO:0006517GO:0005737GO:0004736GO:0008152GO:0004619GO:0046323GO:0016310GO:0006006GO:00163119606P30613P08237P35558P0946710.1038/s41598-018-20348-7null