biostudies-otherUnknownHuman Brainhttps://www.ebi.ac.uk/biostudies/studies/S-BSST1770biostudies-otherfalseCorrelative light and electron microscopy for human brain and other biological models Correlative light and electron microscopy (CLEM) combines light microscopy, for identifying a target via genetic labels, dyes, antibodies, and morphological features, with electron microscopy, for analyzing high-resolution subcellular ultrastructures. Here, we describe the step-by-step instructions to perform a CLEM experiment, optimized for the investigation of ultrastructural features in human brain tissue. The procedure can be carried out at room-temperature, and can be also adapted to other human and animal tissue samples. The procedure requires 8-days to complete and includes the stages of sample fixation for optimal ultrastructural preservation, immunofluorescence staining, image acquisition, multi-modal image correlation, and is executable within standard EM laboratories. Serving as2024-12-20T00:00:00Z2024-12-31T15:38:43.292Z2024-12-19T11:55:07.35ZS-BSST1770