biostudies-otherUnknownJeroen PenningsHorizon Europe Research and Innovation ProgrammeEuropean Union’s Horizon 2020 Research and Innovation ProgrammeHomo sapiens (human)https://www.ebi.ac.uk/biostudies/studies/S-BSST2254biostudies-other101057014964537Jeroen PenningsfalseReactive Oxygen Species formation in HepG2 cells by compound exposureWe quantified ROS formation in HepG2 cells by DCFDA fluorescent probe signal. This was done using five exposure scenarios: (1) HepG2 cells under standard oxygen conditions (20% O2); (2) HepG2 cells under reduced oxygen conditions (3% O2); (3) maturated HepG2 cells receiving acute exposure; (4) maturated HepG2 cells receiving repeated exposure; (5) maturated HepG2 cells receiving repeated exposure and recovery. DCF formation was measured after 1 h of exposure to various compounds at multiple concentrations, as well as several controls. This study was performed as part of the RISK-HUNT3R project and the PARC project.2026-04-30T00:00:00Z2026-04-30T01:01:47.592Z2025-10-28T08:36:57.479ZS-BSST2254