biostudies-otherUnknownUrs MörbeNovo Nordisk FoundationEuropean Crohn’s and Colitis Organisation Lundbeck FoundationLouis-Hansen-FoundationHomo sapiens (human)https://www.ebi.ac.uk/biostudies/studies/S-BSST2281biostudies-otherR155-2014-4184NNF22OC0071681Urs MörbefalseFibroblast diversity within human gut-associated lymphoid tissuesThe provided data include the scRNA-seq data and flow cytometry metadata of the study entitled "Fibroblast diversity within human gut-associated lymphoid tissues", published in the Journal of Experimental Medicine. Procedure for scRNA-seq: Input: Resection material of patients undergoing surgery for colorectal cancer (healthy controls, samples taken >10cm from tumour site and macroscopically tumour free) or IBD resections from the distal ileum. Samples were processed to obtain single cell suspensions as described by Jørgensen et al. (PMID: 33619391). Cells were sorted on a BD Aria cell sorter. After sorting, the cells were subjected to single cell RNA-sequencing using the 10x Genomics platform. scRNA-seq data is provided as read count matrices as well as FASTQ files that were anonymized using the BAMboozled pipeline (PMID: 34711808). FASTQ files: In short, patient identifiable information such as SNPs, insertions and deletions were replaced with the information of the human reference genome to remove person-identifiable information. 2025-11-21T00:00:00Z2026-04-16T19:52:35.52Z2025-11-14T13:27:43.975ZS-BSST2281