{"database":"biostudies-other","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Jeroen Pennings"],"funding":["Horizon Europe Research and Innovation Programme","European Union’s Horizon 2020 Research and Innovation Programme"],"species":["Homo sapiens (human)"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BSST2296"],"repository":["biostudies-other"],"funding_grant_id":["101057014","964537"],"pubmed_authors":["Jeroen Pennings"],"additional_accession":[]},"is_claimable":false,"name":"Malondialdehyde formation in HepG2 cells after compound exposure","description":"We quantified malondialdehyde formation in maturated HepG2 cells after 24 h of compound exposure. This was done using three exposure scenarios: (1) cells receiving acute exposure; (2) cells receiving repeated exposure; (3) cells receiving repeated exposure and recovery. Lipid peroxidation was assessed by measuring malondialdehyde (MDA) levels using the thiobarbituric acid reactive substances (TBARS) assay [Wong et al. Clin Chem. 1987;33(2 Pt1):214-20]. Fluorescence was measured in the supernatant at λexc= 530 nm and λem= 575 nm using a 96-wells fluorescence plate reader. The MDA levels were quantified using a standard curve.\nThis study was performed as part of the RISK-HUNT3R project and the PARC project.","dates":{"release":"2026-03-31T00:00:00Z","modification":"2026-06-29T04:01:37.936Z","creation":"2025-11-20T12:23:14.493Z"},"accession":"S-BSST2296","cross_references":{}}